PMID: 2509483Nov 1, 1989Paper

Intracellular and surface distribution of a membrane protein (CD8) derived from a single nucleus in multinucleated myotubes

The Journal of Cell Biology
E Ralston, Z W Hall

Abstract

We have investigated the contribution of an individual nucleus to intracellular and surface membranes in multinucleated muscle fibers. Using a retroviral vector, we introduced the gene encoding the human T-lymphocyte antigen CD8 into C2 mouse muscle cells to form a stable line expressing the human protein on its surface. The intracellular and surface distributions of the protein were then investigated by immunocytochemistry in hybrid myotubes containing a single nucleus expressing CD8. We show that the intracellular distribution of CD8 is limited to a local area surrounding the nucleus encoding it and several neighboring nuclei. On the cell surface, however, the protein is distributed over the entire myotube. Widespread distribution of a surface membrane protein in multinucleated myotubes can thus result from localized synthesis and processing.

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Citations

Mar 1, 1997·The Anatomical Record·R S HikidaW J Kraemer
Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·H GordonZ W Hall
Dec 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·R KleimanO Steward
Jun 1, 1992·The Journal of Cell Biology·K MetsikköK Väänänen
Dec 1, 1992·The Journal of Cell Biology·E Ralston, Z W Hall
Mar 1, 1997·Developmental Dynamics : an Official Publication of the American Association of Anatomists·C L Pin, P A Merrifield
Aug 10, 2000·Molecular Therapy : the Journal of the American Society of Gene Therapy·L CordierH L Sweeney
Jul 11, 2020·The American Journal of Pathology·Ryan A MartinFrancis X Pizza

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