Intracellular ATP activates inwardly rectifying K+ channels in human and monkey retinal Müller (glial) cells

The Journal of Physiology
S Kusaka, D G Puro

Abstract

1. In the vertebrate retina, the inwardly rectifying K+ (KIR) channels of the Müller (glial) cells are pathways for the redistribution of excess extracellular K+. Due to this role in K+ homeostasis, the activity of Müller cell KIR channels is likely to have significant functional consequences for the retina. In this study we asked whether intracellular ATP regulates the function of KIR channels expressed by Müller cells, the principal glia of the retina. 2. Freshly dissociated Müller cells from the human and monkey (Macaca fascicularis) retina were studied with various configurations of the patch-clamp technique. 3. Whole-cell recordings from Müller cells revealed that a run-down of the inwardly rectifying K+ current (IK(IR)) was prevented if the pipette solution contained Mg-ATP. Chemical ischaemia induced by inhibitors of glycolysis and oxidative phosphorylation caused a nearly 10-fold reduction in the IK(IR)) that was fully restored when metabolically inhibited Müller cells were internally perfused with ATP. 4. In recordings from membrane patches of fresh primate Müller cells, we found that inward-rectifying channels with a conductance of 20 pS in 100 mM Ko+ were the predominant type of KIR channel. In excised patches these ...Continue Reading

Citations

Sep 29, 2005·Acta Ophthalmologica Scandinavica·Andreas BringmannPeter Wiedemann
Mar 11, 2004·The European Journal of Neuroscience·Païkan MarcaggiJonathan A Coles
Oct 26, 2001·Japanese Journal of Pharmacology·Y Horio
Feb 23, 2008·Glia·Yuriy V KucheryavykhSerguei N Skatchkov
May 10, 2007·Journal of Neurophysiology·Sherwin C Lee, Andrew T Ishida
Dec 30, 2014·Physiological Reviews·Francisco V SepúlvedaMaría Isabel Niemeyer
Aug 15, 2001·American Journal of Physiology. Cell Physiology·K HigashiY Kurachi

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