PMID: 9190562May 1, 1997Paper

Intracellular Ca2+ distribution in migrating transformed epithelial cells

Pflügers Archiv : European journal of physiology
Albrecht SchwabH Oberleithner

Abstract

Migration of transformed Madin-Darby canine kidney (MDCK-F) cells depends on the polarized activity of a Ca2+-sensitive K+ channel. We tested whether a gradient of intracellular Ca2+-concentration ([Ca2+]i) underlies the horizontal polarization of K+ channel activity. [Ca2+]i was measured with the fluorescent dye fura-2/AM. Spatial analysis of [Ca2+]i indicated that a horizontal gradient exists, with [Ca2+]i being higher in the cell body than in the lamellipodium. Resting and maximal levels during oscillations of [Ca2+]i in the cell body were found to be 135 +/- 34 and 405 +/- 59 nml/l, respectively, whereas they were 79 +/- 18 and 307 +/- 102 nmol/l in the lamellipodium. This gradient can partially explain the preferential activation of K+ channels in the plasma membrane of the cell body. We applied a local superfusion technique during migration experiments and measurements of [Ca2+]i to test whether its maintenance is due to an uneven distribution of Ca2+ influx into migrating MDCK-F cells. Locally superfusing the cell body of migrating MDCK-F cells with La3+ alone or together with charybdotoxin, a specific blocker of Ca2+-sensitive K+ channels, slowed migration to 47 +/- 10% and 9 +/- 5% of control, respectively. Local block...Continue Reading

Citations

Oct 6, 2006·Pflügers Archiv : European journal of physiology·Albrecht SchwabChristian Stock
Jun 11, 2008·Pflügers Archiv : European journal of physiology·A FabianA Schwab
Dec 2, 2010·Pflügers Archiv : European journal of physiology·Anke FabianAlbrecht Schwab
Apr 25, 2000·The Journal of General Physiology·M KleinA Schwab
Jan 31, 2012·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Nadia K Guimaraes-SouzaJames J Yoo
Jun 1, 2006·Acta Physiologica·C Stock, A Schwab
May 6, 2011·American Journal of Physiology. Cell Physiology·Vishnu Anand Cuddapah, Harald Sontheimer
Aug 27, 2005·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Vladyslav DrevalAlbrecht Schwab
Feb 5, 2014·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Albrecht Schwab, Christian Stock
Jan 9, 2014·Translational Stroke Research·Dongdong ChenLing Wei
Jun 6, 1998·Proceedings of the National Academy of Sciences of the United States of America·J ReinhardtA Schwab
Jan 25, 2014·Pflügers Archiv : European journal of physiology·Lucie ClarysseChristophe Vandier
Apr 7, 2004·The European Journal of Neuroscience·Tom SchillingClaudia Eder
May 10, 2013·Scientific Reports·Patrick HappelIrmgard D Dietzel
Jun 21, 2005·Journal of Cellular Physiology·Albrecht SchwabSteven C Hebert
Jun 11, 2009·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·Min LiBo Wang
May 10, 2003·Pflügers Archiv : European journal of physiology·Robert KraftStephan Patt
May 24, 2019·Cancer Science·Kazuhiro MorishitaHidenori Ichijo
Oct 18, 2012·Physiological Reviews·Albrecht SchwabChristian Stock
Apr 15, 2014·British Journal of Pharmacology·N NielsenA Schwab
Dec 15, 2010·American Journal of Physiology. Cell Physiology·Christine MartinChristian Stock
Apr 9, 2001·American Journal of Physiology. Renal Physiology·A Schwab
Nov 8, 2013·American Journal of Physiology. Cell Physiology·Alban Girault, Emmanuelle Brochiero
Mar 15, 2015·The Journal of Biological Chemistry·Sona Lakshme BalasubramaniamSonali P Barwe
Feb 11, 1998·Physiological Reviews·F LangD Häussinger
Mar 12, 2008·Biochimica Et Biophysica Acta·Liubov Lemberskiy-KuzinZvi Priel

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