Intracellular Ca2+ elevation and contraction due to prostaglandin F2alpha in rat aorta

European Journal of Pharmacology
M TosunR M Rapoport

Abstract

Prostaglandin F2alpha was tested to determine (a) whether its effect on intracellular Ca2+ levels ([Ca2+]i) and force in vascular smooth muscle was mediated through activation of the thromboxane A2 and/or prostaglandin receptor, and (b) the relative roles of Ca2+ influx via L-type and non-L-type Ca2+ channels in prostaglandin receptor-mediated contraction. [Ca2+]i and force were measured simultaneously in fura-2-loaded rat aortic strips. The thromboxane A2 receptor antagonist, SQ29548 ([1S]-1a,2b(5Z),3b,4a-7-(3-[2-[(phenylamino)carbonyl] hydrazinomethyl)-7-oxobicyclo-[2.2.1]hept-2-yl-5-heptenoic acid), prevented the prostaglandin F2alpha-induced plateau [Ca2+]i elevation and force by 80-90%, while abolishing these responses due to the thromboxane A2 receptor agonist, U46619 (9,11-dideoxy-9alpha,11alpha-methanoepoxy prostaglandin F2alpha). Prostaglandin F2alpha (+ SQ29548)-induced plateau [Ca2+]i elevation and force were not inhibited by verapamil. Ni2+, a non-selective cation channel blocker, in the presence of verapamil, abolished the prostaglandin F2alpha (+ SQ29548)-elevated [Ca2+]i, while the contraction was only partially inhibited. These results suggest that, in rat aorta, (1) elevated [Ca2+]i and force due to high prosta...Continue Reading

References

Nov 1, 1995·Journal of Pharmacological and Toxicological Methods·S R MakujinaS J Mustafa

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Citations

May 23, 2001·General Pharmacology·A JednákovitsP P Nánási
Jun 3, 2011·Journal of Biomechanics·André Schmitz, Markus Böl
Dec 5, 2017·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Kemal Gokhan UlusoyOguzhan Yildiz
May 18, 2005·Journal of Cardiovascular Pharmacology·Oguzhan YildizErol Bolu
Dec 19, 2006·American Journal of Physiology. Heart and Circulatory Physiology·M TosunN Karakaya
Nov 7, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Nuria VillalbaDolores Prieto
Nov 10, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Maria V PulinaVera A Golovina
Mar 19, 2003·British Journal of Pharmacology·Richard D JonesKevin S Channer
Mar 13, 2003·American Journal of Physiology. Renal Physiology·Osamu SaitoMatthew D Breyer

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