Intracellular Ca2+ increase induces post-fertilization events via MAP kinase dephosphorylation in eggs of the hydrozoan jellyfish Cladonema pacificum

Developmental Biology
Eri KondohRyusaku Deguchi

Abstract

Naturally spawned eggs of the hydrozoan jellyfish Cladonema pacificum are arrested at G1-like pronuclear stage until fertilization. Fertilized eggs of Cladonema undergo a series of post-fertilization events, including loss of sperm-attracting ability, expression of adhesive materials on the egg surface, and initiation of cell cycle leading to DNA synthesis and cleavage. Here, we investigate whether these events are regulated by changes in intracellular Ca2+ concentration and mitogen-activated protein kinase (MAP kinase) activity in Cladonema eggs. We found that MAP kinase is maintained in the phosphorylated form in unfertilized eggs. Initiation of sperm-induced Ca2+ increase, which is the first sign of fertilization, was immediately followed by MAP kinase dephosphorylation within a few minutes of fertilization. The fertilized eggs typically stopped sperm attraction by an additional 5 min and became sticky around this time. They further underwent cytokinesis yielding 2-cell embryos at approximately 1 h post-fertilization, which was preceded by DNA synthesis evidenced by BrdU incorporation into the nuclei. Injection of inositol 1,4,5-trisphosphate (IP3) into unfertilized eggs, which produced a Ca2+ increase similar to that seen a...Continue Reading

References

Nov 1, 1982·Developmental Biology·G Freeman, R L Miller
Oct 1, 1996·The Journal of Cell Biology·M WildingM Whitaker
Jul 11, 1998·The Journal of Biological Chemistry·M F FavataJ M Trzaskos
Dec 2, 1999·Experimental Cell Research·J EnglishM H Cobb
Dec 10, 1999·Developmental Biology·M C Gould, J L Stephano
May 17, 2000·Molecular Reproduction and Development·M MarinoB Dale
Jun 6, 2000·Developmental Biology·J L Stephano, M C Gould
Aug 17, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·K Roovers, R K Assoian
Dec 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·K TachibanaT Kishimoto
Feb 13, 2001·Seminars in Cell & Developmental Biology·L A JaffeK R Foltz
Oct 3, 2001·International Review of Cytology·G M WesselS Conner
May 22, 2002·Cellular Signalling·Neus AgellPriam Villalonga
Nov 2, 2002·Proceedings of the National Academy of Sciences of the United States of America·Manabu YoshidaMasaaki Morisawa
Mar 26, 2003·Genes & Development·Brian J Tunquist, James L Maller
Aug 28, 2003·Biology of the Cell·Amelia Portillo-LópezJosé Luis Stephano
Dec 13, 2003·Endocrine Reviews·Christina R Kahl, Anthony R Means
May 12, 2004·Developmental Biology·Levent TutuncuCarmen J Williams
Oct 30, 2004·Molecular Reproduction and Development·T L Smythe, S A Stricker

❮ Previous
Next ❯

Citations

Apr 12, 2014·Biochemical and Biophysical Research Communications·Vlad CostacheRémi Dumollard
Aug 1, 2013·Molecular Reproduction and Development·Junaid KashirStephen A Stricker
Jul 21, 2011·Molecular Reproduction and Development·Ryusaku DeguchiStephen A Stricker
May 23, 2012·Developmental Biology·Noburu SensuiKazunori Tachibana
May 9, 2014·Developmental Biology·Mio ArakawaRyusaku Deguchi
Jun 25, 2009·Current Biology : CB·Cassandra Extavour
Feb 24, 2009·Current Biology : CB·Aldine AmielEvelyn Houliston
Apr 24, 2014·The Journal of Biological Chemistry·Ru-Bing DuanWei-Jun Yang
Apr 17, 2009·Development·Masatoshi HaraTakeo Kishimoto
Oct 1, 2013·Journal of Cell Science·Nathalie OulhenRemi Dumollard
Apr 27, 2013·The Journal of Experimental Biology·Daisuke S YamamotoHiroyuki Matsuoka

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Anthelmintics (ASM)

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.

Anthelmintics

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.