Intracellular Ca2+ regulating proteins in vascular smooth muscle cells are altered with type 1 diabetes due to the direct effects of hyperglycemia.

Cardiovascular Diabetology
Yvonne M SearlsLisa Stehno-Bittel

Abstract

Diminished calcium (Ca2+) transients in response to physiological agonists have been reported in vascular smooth muscle cells (VSMCs) from diabetic animals. However, the mechanism responsible was unclear. VSMCs from autoimmune type 1 Diabetes Resistant Bio-Breeding (DR-BB) rats and streptozotocin-induced rats were examined for levels and distribution of inositol trisphosphate receptors (IP3R) and the SR Ca2+ pumps (SERCA 2 and 3). Generally, a decrease in IP3R levels and dramatic increase in ryanodine receptor (RyR) levels were noted in the aortic samples from diabetic animals. Redistribution of the specific IP3R subtypes was dependent on the rat model. SERCA 2 was redistributed to a peri-nuclear pattern that was more prominent in the DR-BB diabetic rat aorta than the STZ diabetic rat. The free intracellular Ca2+ in freshly dispersed VSMCs from control and diabetic animals was monitored using ratiometric Ca2+ sensitive fluorophores viewed by confocal microscopy. In control VSMCs, basal fluorescence levels were significantly higher in the nucleus relative to the cytoplasm, while in diabetic VSMCs they were essentially the same. Vasopressin induced a predictable increase in free intracellular Ca2+ in the VSMCs from control rats w...Continue Reading

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Citations

Mar 29, 2011·Cold Spring Harbor Perspectives in Biology·Djalila MekahliLudwig Missiaen
Mar 27, 2012·American Journal of Physiology. Heart and Circulatory Physiology·Damodaran NarayananJonathan H Jaggar
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Mar 10, 2020·Clinical and Experimental Pharmacology & Physiology·Christian SchachStefan Wagner
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Jul 1, 2020·Cellular and Molecular Life Sciences : CMLS·Madeline Nieves-CintrónManuel F Navedo
Feb 21, 2014·F1000Research·Shaimaa N AminIbrahim Mohamady

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