PMID: 9185165Jul 1, 1997Paper

Intracellular Ca2+ signals induced by ATP and thapsigargin in glioma C6 cells. Calcium pools sensitive to inositol 1,4,5-trisphosphate and thapsigargin

Neurochemistry International
Paweł SabałaJolanta Barańska

Abstract

In glioma C6 cells, extracellular ATP generates inositol 1,4,5-trisphosphate (InsP3), indicating the presence of purinergic receptors coupled to phosphoinositide turnover. To identify the effect of ATP (acting via InsP3) and thapsigargin (acting without InsP3 production as a specific inhibitor of the endoplasmic reticulum Ca(2+)-ATPase) on intracellular Ca2+ pools we used video imaging of Fura-2 loaded into single, intact glioma C6 cells. It has been shown that ATP and thapsigargin initiate Ca2+ response consistent with the capacitative model of Ca2+ influx. When the cells were stimulated by increasing concentrations of ATP (1, 10, 50 and 100 microM) the graded, quantal Ca2+ response was observed. In the absence of extracellular Ca2+ thapsigargin and ionomycin-releasable Ca2+ pools are overlapping, demonstrating that Ca2+ stores are located mainly in the endoplasmic reticulum. After maximal Ca2+ mobilization by ATP, thapsigargin causes further increase in cytosolic Ca2+ concentration, whereas emptying of thapsigargin-sensitive intracellular stores prevents any further Ca2+ release by ATP. Thus, the thapsigargin-sensitive intracellular pool of Ca2+ in glioma C6 cells seems to be larger than that sensitive to InsP3. Two hypothesi...Continue Reading

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Citations

Jun 26, 2013·Purinergic Signalling·Geoffrey Burnstock, Francesco Di Virgilio
Jun 18, 2002·European Journal of Pharmacology·Robert MellerTrevor Sharp
Apr 11, 2009·Archives of Histology and Cytology·Afadhali Denis RussaYoh-ichi Satoh
Aug 7, 2002·Biochemical and Biophysical Research Communications·Pawelz SabałaJolanta Barańska
Jul 15, 1999·Biochemical and Biophysical Research Communications·R Czajkowski, J Barańska
Aug 6, 2005·Neurochemistry International·Katrin Sak, Peter Illes

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