Intracellular calcium as a second messenger following growth stimulation of human keratinocytes

The British Journal of Dermatology
U B McGovernG R Sharpe

Abstract

The mitogenic effect of the neuropeptide substance P and bombesin was investigated in normal human keratinocytes in serum-free culture, both with and without the presence of epidermal growth factor (EGF). Although both neuropeptides induced a small increase in cell numbers in the presence of EGF, the response was much greater in its absence, and cell numbers increased to 200% of controls at 5 days. Changes in intracellular free calcium are frequently seen following mitogenic stimulation of cells, and this phenomenon was studied in individual keratinocytes. Epidermal growth factor (10 ng/ml) induced calcium transients in 57% (n = 21) of cells. The mean intracellular free calcium was 97 +/- 11 nM (mean +/- SEM) in quiescent cells, and the calcium transients reached approximately 250 nM for 3-4 min. In the presence of EGF, calcium transients were never observed with the addition of either substance P or bombesin. For EGF-deprived cultures, 20% of keratinocytes (n = 10) showed a large calcium transient following the addition of 500 nM bombesin, and 63% (n = 12) of cells gave calcium transients following the addition of 700 nM of substance P. Studies in calcium-free medium, and following depletion of intracellular calcium stores wit...Continue Reading

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Citations

Aug 24, 1999·British Journal of Pharmacology·C J DixonJ A Gallagher
Apr 23, 2002·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Yuji YamaguchiSatoshi Itami
Mar 29, 2005·Experimental Biology and Medicine·Angel V DelgadoJames P Chambers
Dec 17, 2009·Journal of Cutaneous Pathology·Nils WeigeltSonja Ständer
Oct 4, 2006·Physiological Reviews·Dirk RoostermanMartin Steinhoff
Sep 26, 2000·American Journal of Physiology. Cell Physiology·B X ZhangM S Katz
Oct 19, 2013·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Jérémy ChéretChristelle Le Gall-Ianotto
May 1, 2007·Pain·Florence H MujendaGary R Strichartz
Nov 17, 2007·The Journal of Investigative Dermatology·Helen E BurrellJames A Gallagher

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