Intracellular calcium requirements for beta1 integrin activation

Journal of Cellular Physiology
M E RowinJ F Bohnsack

Abstract

Human polymorphonuclear leukocytes (PMNs) express beta1 integrins that mediate adhesion to extracellular matrix proteins following stimulation with agonists that induce an increase in intracellular calcium. The purpose of these studies was to determine the contribution made by alterations in intracellular calcium ([Ca++]i) to inside-out activation of beta1 integrins using dimethyl sulfoxide (DMSO)-differentiated granulocytic HL60 cells as a model of human PMNs. Activation of beta1 integrins was determined by measuring the expression of an activation-dependent epitope on the beta1 subunit that is recognized by monoclonal antibody (mAb) 15/7. Exposure of granulocytic HL60 cells to calcium ionophore ionomycin (800 nM) alone did not increase the binding of mAb 15/7 to the cell surface, nor did it increase beta1 integrin-mediated adhesion of the cells to fibronectin. Similarly, exposure of the cells to the direct protein kinase C (PKC) activator, dioctanoylglycerol (di-C8) at 100 microM, neither increased binding of mAb 15/7 to these cells nor adhesion to fibronectin. Simultaneous addition of di-C8 and ionomycin, however, caused a significant increase in the expression of the 15/7 epitope and cell adhesion, suggesting synergy betwee...Continue Reading

References

May 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·S J CollinsR C Gallo
Nov 1, 1992·The Journal of Clinical Investigation·B J HughesC W Smith
Dec 1, 1992·The Biochemical Journal·D C AltieriC G Gahmberg
May 1, 1992·The Journal of Cell Biology·A G ArroyoF Sánchez-Madrid
Jan 1, 1991·The Journal of Cell Biology·Y van KooykC G Figdor
Jun 1, 1991·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Y Shimizu, S Shaw
Apr 1, 1990·The Journal of Experimental Medicine·J F BohnsackG A Zimmerman
Nov 15, 1993·Biochemical and Biophysical Research Communications·P J HartfieldR D Camp
Apr 1, 1995·Journal of Leukocyte Biology·J F BohnsackT A Yednock
Mar 1, 1993·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S L ErlandsenR D Nelson

❮ Previous
Next ❯

Citations

Jul 1, 2004·The Journal of Biological Chemistry·Gordon J ZwartzLarry A Sklar
Jan 22, 2005·Journal of Leukocyte Biology·Alejandro Ortiz-Stern, Carlos Rosales
Mar 24, 2018·Arteriosclerosis, Thrombosis, and Vascular Biology·Martina RamiSabine Steffens
Aug 20, 2005·Journal of Biomedical Materials Research. Part a·Anuradha Subramanian, Hsin-Yi Lin
Aug 28, 2020·Frontiers in Physiology·Francesca AglialoroEmile van den Akker
Mar 29, 2011·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Amalia VartanianMikhail Lichinitser
Jan 15, 2002·American Journal of Physiology. Cell Physiology·Roger BelusaAnita Aperia
Jun 16, 2016·Frontiers in Physiology·Sujeenthar Tharmalingam, David R Hampson
May 1, 2005·Journal of Leukocyte Biology·Alejandro Ortiz-Stern, Carlos Rosales
Jan 2, 2009·Journal of Cell Science·Maria Rosaria CeraElisabetta Dejana
Feb 5, 2004·Archives of Biochemistry and Biophysics·Nicholas G GenesLawrence J Bonassar
Jun 15, 2021·American Journal of Physiology. Renal Physiology·Diana Hamdan, Lisa A Robinson

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.