PMID: 9429762Jan 16, 1998Paper

Intracellular calcium response of ACL and MCL ligament fibroblasts to fluid-induced shear stress

Cellular Signalling
C T HungP A Torzilli

Abstract

This study examines the real-time intracellular calcium concentration, [Ca2+]i, response of canine medial collateral ligament (MCL) and anterior cruciate ligament (ACL) fibroblasts subjected to a fluid-induced shear stress of 25 dynes/cm2. In experiments using a modified Hanks' Balanced Salt Solution (HBSS) perfusate, both cell types demonstrated a significant increase in peak [Ca2+]i compared to respective no-flow controls, the response of MCL fibroblasts being nearly 2-fold greater than that of ACL fibroblasts. In studies where the cells were bathed in a medium of HBSS supplemented with 2% newborn bovine serum (NBS) and then introduced to flow with the same medium, ACL fibroblasts responded nearly 3-fold greater than MCL fibroblasts. Neomycin (10 mM), thapsigarigin (1 microM) and Ca(2+)-free media supplemented with EGTA (1 mM) were able to inhibit significantly the [Ca2+]i response to flow with HBSS in both fibroblasts. Thapsigargin also blocked the NBS flow response in both cell types, while neomycin and Ca(2+)-free media significantly inhibited the ACL response. Our findings demonstrate that ACL and MCL cells are not the same. These differences may be related to the disparate healing capacity of the ACL and MCL observed cli...Continue Reading

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Citations

Jun 22, 1999·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·F GuilakH J Donahue
Dec 12, 2012·Cell and Tissue Research·Nickolay V BukoreshtlievAndrew E Pelling
Feb 23, 2002·Journal of Biomechanics·Joanne M ArchambaultAlbert J Banes
May 9, 2012·Proceedings of the National Academy of Sciences of the United States of America·Bo SunHoward A Stone
Jan 19, 2013·Tissue Engineering. Part a·Thomas K H TehJames C H Goh
Sep 11, 2002·Cell Structure and Function·Sung-Gon KimHisashi Kurosawa
May 13, 2008·Journal of Biomedical Materials Research. Part a·Qingjun Kong, Maribel Vazquez
Oct 31, 2007·Journal of Biomedical Materials Research. Part a·S ScaglioneI Martin
Oct 5, 2010·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Vonda WrightJo A Hannafin
Jun 3, 2010·Biotechnology and Bioengineering·Liu DanKah-Fai Leong
Jul 25, 2006·Acta Biomaterialia·Tumul Gupta, Tammy L Haut Donahue
Jun 13, 2015·Journal of the Royal Society, Interface·Josephine LembongHoward A Stone
May 3, 2005·Journal of Biomechanics·P Grace ChaoClark T Hung
Mar 12, 2004·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Steven P ArnoczkyKeri Gardner
Aug 31, 2001·Journal of Cellular Biochemistry·M K ElfervigA J Banes
Sep 6, 2005·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Ranjan GuptaClark T Hung
Feb 16, 2006·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Robert L EiflerTammy L Haut Donahue
May 2, 2003·The Journal of Comparative Neurology·Ranjan Gupta, Oswald Steward
Feb 24, 2006·The Biochemical Journal·Jennifer R MackleySteven J Winder
Aug 25, 2001·Osteoarthritis and Cartilage·M K ElfervigA J Banes

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