Intracellular cAMP increases during the positive inotropism induced by androgens in isolated left atrium of rat

European Journal of Pharmacology
Lucía VelascoBegoña Cantabrana

Abstract

Molecular interactions of androgens with the plasma membrane may produce rapid cardiovascular effects that cannot be explained by the classic genomic mechanisms. In this sense, 5 alpha- and 5 beta-dihydrotestosterone-induced an acute positive inotropic effect in isolated left atrium of rat, an effect which may be due to cAMP-dependent mechanisms. To prove this, intracellular levels of cAMP, after exposure to androgens in the organ bath, and binding to beta(1)-adrenoceptors were evaluated. After a 4-min exposure, 5 alpha- and 5 beta-dihydrotestosterone increased cAMP levels from 3.83+/-0.61 to 6.15+/-1.1 and 11.18+/-2.4 pmol cAMP/mg of protein, respectively. These increases were inhibited by atenolol and not modified by treatment of the rats with reserpine. The androgen-induced cAMP increase seems to be produced via an extracellular interaction, because positive inotropism and raised levels of cAMP were produced by 5 alpha-dihydrotestosterone conjugated with bovine serum albumin (BSA). In addition, it is independent of beta(1)-adrenoceptor activation, because neither androgen displaced [(3)H]dihydroalprenolol binding. Therefore, the androgens induced a positive inotropic effect via a postsynaptic effect that increases intracellu...Continue Reading

References

Jan 1, 1992·Annual Review of Biochemistry·P C Lucas, D K Granner
Oct 1, 1992·Trends in Pharmacological Sciences·W Schütz, M Freissmuth
Jan 1, 1988·Methods in Enzymology·L H BotelhoB Jastorff
Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·E J LewisD M Chikaraishi
Aug 1, 1986·Molecular and Cellular Biology·T BarkaH M van der Noen
Feb 15, 1995·Circulation·P YueP Collins
Jul 15, 1994·European Journal of Pharmacology·K Götze, K H Jakobs
Aug 30, 1994·Proceedings of the National Academy of Sciences of the United States of America·S M AronicaB S Katzenellenbogen
Jan 1, 1993·Annual Review of Biochemistry·A KolbS Adhya
Oct 8, 1997·The American Journal of Physiology·A R Patel, J Y Wang

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Citations

May 17, 2014·Substance Use & Misuse·Marcos Antonio Pereira dos SantosAlexandre Sérgio Silva
Jan 13, 2015·Steroids·Figueroa-Valverde LauroSarabia Alcocer Betty
Oct 24, 2018·Europace : European Pacing, Arrhythmias, and Cardiac Electrophysiology : Journal of the Working Groups on Cardiac Pacing, Arrhythmias, and Cardiac Cellular Electrophysiology of the European Society of Cardiology·Katja E OdeningHikmet Yorgun
Aug 2, 2008·Journal of Cardiovascular Pharmacology·Lucía VelascoManuel Sánchez

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