PMID: 9446708Apr 4, 1998Paper

Intracellular cAMP potentiates voltage-dependent activation of L-type Ca2+ channels in rat islet beta-cells

Pflügers Archiv : European journal of physiology
T KannoM Wakui

Abstract

Intracellular cAMP-dependent modulation of L-type Ca2+ channel activation in cultured rat islet beta-cells has been investigated using the patch-clamp whole-cell current recording mode. The L-type voltage-dependent Ca2+ current (ICa) showed a fast activation followed by a slow inactivation, and was sensitive to Ca2+ channel blockers, for example nifedipine. Application of a cAMP analogue, dibutyryl cyclic AMP (db-cAMP), increased the magnitude of the peak ICa in a concentration-dependent manner. Values of the half-activation potentials (V1/2), taken from activation curves for ICa, were -16.7 +/- 1.8 and -21.9 +/- 3.4 mV (P < 0.05) before and after application of db-cAMP, respectively, with no change of the slope factor (k) or the reversal potential. Pretreatment with a specific protein kinase A antagonist, Rp-cAMP, prevented the potentiating effect of db-cAMP. These results indicate that in rat islet beta-cells, phosphorylation of cAMP-dependent kinase potentiates the voltage-dependent activation of L-type Ca2+ channels.

Citations

Feb 26, 2003·The Journal of General Physiology·Lena EliassonPatrik Rorsman
Apr 28, 2012·Molecular Endocrinology·David M KellerRichard H Goodman
Feb 1, 2002·Biochemical and Biophysical Research Communications·Giuliano MeyerMarkus Paulmichl
Jul 24, 2014·Cellular and Molecular Life Sciences : CMLS·Shao-Nian YangPer-Olof Berggren
Sep 14, 2012·Upsala Journal of Medical Sciences·Anders Tengholm
Jul 21, 2016·Molecular Pharmacology·Myrian VelascoMarcia Hiriart
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Feb 13, 2021·Antioxidants·Petr JežekLydie Plecitá-Hlavatá
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Sep 13, 2011·Progress in Biophysics and Molecular Biology·Jae-Hyung ParkDae-Kyu Song

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