PMID: 513117Nov 30, 1979Paper

Intracellular chloride activities and active chloride absorption in the intestinal epithelium of the winter flounder

The Journal of Membrane Biology
M E DuffeyS G Schultz

Abstract

Intracellular chloride activities, (Cl)c, and the electrical potential difference across the mucosal membrane, psimc, were determined in the isolated small intestine of the winter flounder, using Cl-selective and conventional (KCl-filled) microelectrodes. In the presence of a Na-containing buffer psimc averages -69 mV and (Cl)c averages 24 mM, a value that is 3.4 times that predicted for an equilibrium distribution across the mucosal membrane. On the other hand, when the tissue is then perfused with Na-free buffer, (Cl)c slowly falls to a value that does not differ significantly from that predicted for an equilibrium distribution, and psimc depolarizes significantly. Finally, when the tissue is again bathed in the Na-containing buffer, (Cl)c rapidly returns to a value well above equilibrium. These results, together with those of Frizzell et al. (J. Membrane Biol. 46:27, 1979), provide direct evidence that: (1) Cl is accumulated against its electrochemical potential difference (32 mV) by this tissue, and (2) this accumulation is coupled to and energized by the entry of Na down its steep electrochemical potential difference.

References

Jun 1, 1975·The Journal of General Physiology·R A FrizzellS G Schultz
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Jan 1, 1979·The American Journal of Physiology·T Akiyama, H A Fozzard
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Aug 1, 1973·The American Journal of Physiology·H N NellansS G Schultz
Jan 2, 1973·Biochimica Et Biophysica Acta·R A FrizzellS G Schultz

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Citations

Jan 1, 1982·The Journal of Membrane Biology·U KatzJ C Ellory
Nov 1, 1991·The Journal of Membrane Biology·S M O'GradyJ L Rae
Jan 1, 1982·The Journal of Membrane Biology·M M Civan, R J Bookman
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Jan 1, 1981·The Journal of Membrane Biology·K U PetersenK Heintze
Jan 1, 1984·Comparative Biochemistry and Physiology. A, Comparative Physiology·D CremaschiM W Smith
Apr 1, 1992·Pflügers Archiv : European journal of physiology·A De GiorgiA Corcelli
Aug 1, 1992·Kidney International·R D LondonR G Abramson
Jul 27, 1983·Biochimica Et Biophysica Acta·C M FestenC H Van Os
Sep 18, 1987·Biochimica Et Biophysica Acta·V LyallW M Armstrong
Nov 25, 1982·Nature·M W MuschR A Frizzell
Jan 1, 1984·The Journal of Membrane Biology·W Almers, C Stirling
Jan 1, 1996·Critical Reviews in Biochemistry and Molecular Biology·G A Gerencser
Oct 22, 2002·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Ryan M Pelis, J Larry Renfro
Jun 30, 2016·Cells·Florian Geisler, Rudolf E Leube
Jan 1, 1986·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·J M Dixon, C A Loretz
Jan 1, 1982·The Journal of Membrane Biology·W B GugginoG Giebisch
Jan 1, 1981·The Journal of Membrane Biology·A EdelmanT Anagnostopoulos
Feb 1, 1990·The American Journal of Physiology·S M O'Grady, P J Wolters
Jul 22, 2006·The Journal of Experimental Biology·Martin Grosell

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