PMID: 8584420Nov 1, 1995Paper

Intracellular Cl- modulates Ca2+-induced exocytosis from rat melanotrophs through GTP-binding proteins

Pflügers Archiv : European journal of physiology
M Rupnik, R Zorec

Abstract

We used the whole-cell patch-clamp technique to monitor changes in membrane capacitance (Cm) to study the influence of cytosolic concentration ([Cl-]i) on the secretory activity of rat melanotrophs. The sensitivity of the secretory machinery to Ca2+ was enhanced in the presence of a high [Cl-]i. The free concentration of Ca2+ required for half-maximal secretory activity was reduced from 3.2microM at 4mM [Cl-]i to 0.7microM at 154mM [Cl-]i. To study whether the modulation of secretory activity by Cl- involves guanosine 5'-triphosphate-(GTP-) binding proteins, cells were dialysed with non-hydrolysable GTP and GDP analogues, fluoroaluminate (AlF4(-)), or were pretreated with pertussis toxin. With guanosine 5'-O-(3-thiotriphosphate) (GTP[gamma-S], 100microM) the maximal rate of Cm increase (dCm/dt) was enhanced at 4 and 14mM [Cl-]i, but it was not affected at 154mM [Cl-]i. In contrast, the secretory response, measured as a percentage of resting Cm 10min after the start of recordings, was reduced at 154mM [Cl-]i, but not affected at 4mM [Cl-]i. Only with 154mM [Cl-]i did intracellular dialysis of cells with guanosine 5'-O-(2-thiodiphosphate) (GDP[beta-S], 500microM) inhibit dCm/dt as well as relative secretory responses. The presenc...Continue Reading

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Citations

Jan 1, 1997·Progress in Biophysics and Molecular Biology·B NiliusG Droogmans
Sep 23, 2003·Brain Research. Molecular Brain Research·Nathalie GriffonPierre Sokoloff
Jul 24, 2010·Endocrine Reviews·Stanko S StojilkovicRichard Bertram
May 3, 2000·Proceedings of the National Academy of Sciences of the United States of America·M RupnikR Zorec
Dec 1, 1996·Trends in Endocrinology and Metabolism : TEM·R Zorec
Jul 11, 2006·American Journal of Physiology. Cell Physiology·M RupnikR Zorec
Oct 22, 2002·American Journal of Physiology. Cell Physiology·M KreftR Zorec
Aug 4, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·Chikao ShimamotoTakashi Nakahari
Jul 23, 2011·Physiological Research·J DolenšekM S Rupnik
Mar 8, 2000·Biochimica Et Biophysica Acta·W B Thoreson, S L Stella
Dec 26, 2001·Biophysical Journal·Igor PoberajRobert Zorec

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