Intracellular concentrations of Ca(2+) modulate the strength of signal and alter the outcomes of cytotoxic T-lymphocyte antigen-4 (CD152)-CD80/CD86 interactions in CD4(+) T lymphocytes.

Immunology
Asma AhmedDipankar Nandi

Abstract

The costimulatory receptors CD28 and cytotoxic T-lymphocyte antigen (CTLA)-4 and their ligands, CD80 and CD86, are expressed on T lymphocytes; however, their functional roles during T cell-T cell interactions are not well known. The consequences of blocking CTLA-4-CD80/CD86 interactions on purified mouse CD4(+) T cells were studied in the context of the strength of signal (SOS). CD4(+) T cells were activated with phorbol 12-myristate 13-acetate (PMA) and different concentrations of a Ca(2+) ionophore, Ionomycin (I), or a sarcoplasmic Ca(2+) ATPase inhibitor, Thapsigargin (TG). Increasing concentrations of I or TG increased the amount of interleukin (IL)-2, reflecting the conversion of a low to a high SOS. During activation with PMA and low amounts of I, intracellular concentrations of calcium ([Ca(2+)](i)) were greatly reduced upon CTLA-4-CD80/CD86 blockade. Further experiments demonstrated that CTLA-4-CD80/CD86 interactions reduced cell cycling upon activation with PMA and high amounts of I or TG (high SOS) but the opposite occurred with PMA and low amounts of I or TG (low SOS). These results were confirmed by surface T-cell receptor (TCR)-CD3 signalling using a low SOS, for example soluble anti-CD3, or a high SOS, for example...Continue Reading

References

Aug 1, 1995·The Journal of Experimental Medicine·M F Krummel, J P Allison
Aug 1, 1994·Immunity·T L WalunasJ A Bluestone
Jan 6, 1999·Proceedings of the National Academy of Sciences of the United States of America·P A Bretscher
Jul 21, 1999·Proceedings of the National Academy of Sciences of the United States of America·C A ChambersJ P Allison
Oct 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·M S KuhnsJ P Allison
Mar 10, 2001·Immunity·R J GreenwaldA H Sharpe
Mar 13, 2001·Annual Review of Immunology·R S Lewis
Jun 1, 2001·European Journal of Immunology·W LiuJ O Liu
Jun 28, 2002·Nature Immunology·Jackson G EgenJames P Allison
Nov 22, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lucy S K WalkerAbul K Abbas
Nov 22, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Silvia VendettiMaria Teresa De Magistris
Nov 26, 2002·Nature Immunology·Michael Reth
Apr 30, 2003·Nature Immunology·Marja Jäättelä, Jürg Tschopp
Jul 3, 2003·Nature Reviews. Molecular Cell Biology·Sten OrreniusPierluigi Nicotera
Sep 17, 2003·Genes & Development·Patrick G HoganAnjana Rao
Dec 23, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Patricia A TaylorBruce R Blazar
May 7, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Joaquín MadrenasVincent Ling
Jul 9, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Philip H J RemansKris A Reedquist
Sep 11, 2004·Immunity·Tsvetelina Pentcheva-HoangJames P Allison
Sep 29, 2004·Free Radical Biology & Medicine·Mark S Williams, Jaeyul Kwon
Nov 24, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hyokjoon KwonJi-Won Yoon
Jan 13, 2006·Immunology and Cell Biology·Axel J HueberThomas Nagel
Mar 23, 2006·Annual Review of Immunology·Wendy A TeftJoaquín Madrenas
Oct 25, 2006·Proceedings of the National Academy of Sciences of the United States of America·Zhibin ChenChristophe Benoist
Dec 21, 2007·European Journal of Immunology·Helga SchneiderChristopher E Rudd
Jan 26, 2008·Nature Reviews. Immunology·Christopher E Rudd

❮ Previous
Next ❯

Citations

Dec 21, 2012·Immunology Letters·Sandeep KumarPremendra D Dwivedi
Apr 2, 2011·International Journal of Cancer. Journal International Du Cancer·Srabanti RakshitDipankar Nandi
Mar 26, 2013·Allergology International : Official Journal of the Japanese Society of Allergology·Tomotaka KawayamaTomoaki Hoshino
Jun 1, 2018·Journal of Immunology Research·Xian ChenFang Wang
Jan 6, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sambuddho MukherjeeJo Rae Wright

❮ Previous
Next ❯

Related Concepts

Related Feeds

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.