Intracellular delivery of molecules using microfabricated nanoneedle arrays

Biomedical Microdevices
Seonhee ParkMark R Prausnitz

Abstract

Many bioactive molecules have intracellular targets, but have difficulty crossing the cell membrane to reach those targets. To address this difficulty, we fabricated arrays of nanoneedles to gently and simultaneously puncture 10(5) cells and thereby provide transient pathways for transport of molecules into the cells. The nanoneedles were microfabricated by etching silicon to create arrays of nanoneedles measuring 12 μm in height, tapering to a sharp tip less than 30 nm wide to facilitate puncture into cells and spaced 10 μm apart in order to have at least one nanoneedle puncture each cell in a confluent monolayer. These nanoneedles were used for intracellular delivery in two ways: puncture loading, in which nanoneedle arrays were pressed into cell monolayers, and centrifuge loading, in which cells in suspension were spun down onto nanoneedle arrays. The effects on intracellular uptake and cell viability were determined as a function of nanoneedle length and sharpness, puncture force and duration, and molecular weight of the molecule delivered. Under optimal conditions, intracellular uptake was seen in approximately 50 % of cells while maintaining high cell viability. Overall, this study provides a comparative analysis of intra...Continue Reading

References

Mar 15, 1978·International Journal of Cancer. Journal International Du Cancer·K R StoneD F Paulson
Feb 13, 2001·Biophysical Journal·P J CanatellaM R Prausnitz
Jul 13, 2002·Journal of Pharmaceutical Sciences·Héctor R GuzmánMark R Prausnitz
Nov 19, 2003·Proceedings of the National Academy of Sciences of the United States of America·Devin V McAllisterMark R Prausnitz
Jul 1, 2004·PLoS Biology·Jyoti K JaiswalSanford M Simon
Jun 21, 2006·Ultrasound in Medicine & Biology·Robyn K SchlicherMark R Prausnitz
Jul 13, 2006·Annual Review of Biomedical Engineering·Vladimir P Torchilin
May 9, 2007·Proceedings of the National Academy of Sciences of the United States of America·Xing ChenCarolyn R Bertozzi
May 23, 2007·Journal of the American Chemical Society·Woong KimPeidong Yang
Jul 25, 2008·Lab on a Chip·Andrea Adamo, Klavs F Jensen
Aug 21, 2008·British Journal of Anaesthesia·M J SheenS-T Huang
Aug 30, 2008·Current Opinion in Biotechnology·Yan Zhang, Long-Chuan Yu
Mar 4, 2009·Nano Letters·Sangjin ParkSangyong Jon
Jan 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Alex K ShalekHongkun Park
May 12, 2012·Advanced Drug Delivery Reviews·Yeu-Chun KimMark R Prausnitz
Mar 1, 2013·Advanced Healthcare Materials·Xianfeng ChenWenjun Zhang
Jul 3, 2013·Journal of Laboratory Automation·J Mark MeachamAndrei G Fedorov
Apr 16, 2014·Journal of Nanoscience and Nanotechnology·Sung-Woong HanTaiji Adachi
Jul 16, 2014·Nature Reviews. Genetics·Hao YinDaniel G Anderson
Jul 30, 2014·Nature Communications·Ying WangPeng Shi
Aug 30, 2014·Small·Li YanXianfeng Chen

❮ Previous
Next ❯

Citations

Feb 23, 2019·Nanotechnology·Apresio K Fajrial, Xiaoyun Ding
Jan 17, 2020·Advanced Materials·Stuart G HigginsMolly M Stevens
Oct 31, 2017·Biomedical Optics Express·Nabiha SaklayenEric Mazur
Feb 4, 2021·Microsystems & Nanoengineering·An'an ShengHuanyu Cheng
Jun 9, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Michele BecceMolly M Stevens
Jun 20, 2020·ACS Applied Materials & Interfaces·Yangcui QuHong Chen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Canadian Medical Association Journal
A T Cameron
Conference Proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society
Jung-Hwan ParkMark R Prausnitz
© 2022 Meta ULC. All rights reserved