PMID: 7027260Jul 1, 1981Paper

Intracellular disruption of rat heart lysosomes by leucine methyl ester: effects on protein degradation

Proceedings of the National Academy of Sciences of the United States of America
J P ReevesK Wildenthal

Abstract

Perfusion of rat hearts with Krebs--Henseleit medium containing 10 mM L-leucine methyl ester leads to swelling of lysosomes and loss of lysosomal integrity within 30-60 min. No morphological changes can be detected in the nuclei, mitochondria, sarcoplasmic reticulum, or Golgi complex as a result of the treatment with leucine methyl ester, and the hearts continue to beat normally during the treatment period. Homogenates of rat hearts perfused with the methyl ester exhibit a decrease in the sedimentability of cathepsin D activity compared to controls, thus providing additional evidence for a loss of lysosomal integrity. Swelling and disruption of the lysosomes presumably occurs because of the extensive accumulation of leucine within the organelles resulting from the intralysosomal hydrolysis of the freely permeating methyl ester. The lysosomal dysfunction that occurs with exposure to leucine methyl ester produces a 30% decrease in cardiac protein degradation. These results provide an estimate of the contribution of lysosomes to total protein degradation in the rat heart, and they also suggest that the enzymes released as a result of lysosomal disruption are relatively inactive in hydrolyzing cellular constituents under the perfus...Continue Reading

References

Mar 15, 1979·Biochemical and Biophysical Research Communications·W F WardG E Mortimore
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Aug 22, 1973·Biochimica Et Biophysica Acta·R Goldman, A Kaplan
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Jun 15, 1961·Proceedings of the National Academy of Sciences of the United States of America·A B NOVIKOFF, S GOLDFISCHER

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Citations

Mar 1, 1992·Biochimie·D E GollJ A Christiansen
Feb 13, 1989·Neuroscience Letters·Y NakamuraT Nishimura
Dec 1, 1983·The Journal of Cell Biology·D K MillerR A Firestone
Sep 1, 1990·Cell and Tissue Kinetics·I B Rosenwald
Mar 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·M F PfisterH Murer
Feb 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·N G SeidahM Marcinkiewicz
Nov 1, 1988·Immunology Today·C A Borrebaeck
Jan 1, 1988·Immunology Today·D L Thiele, P E Lipsky
Jan 1, 1989·Progress in Histochemistry and Cytochemistry·G Rappay
Aug 1, 1986·Metabolism: Clinical and Experimental·R Odessey
Jan 26, 2007·Acta Tropica·Camila M AdadeMaurilio J Soares
Aug 1, 1984·Journal of Neurochemistry·R A Nixon, C A Marotta
Aug 1, 1985·Acta Pharmacologica Et Toxicologica·F FranconiA Giotti
Feb 1, 1993·Clinical and Experimental Immunology·M E Alarcón-RiquelmeC Fernández
Mar 1, 1983·The American Journal of Physiology·B A BrooksG Hennecke

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