PMID: 9177056Jan 1, 1997Paper

Intracellular distribution and identification of the nuclear localization signals of two plant heat-stress transcription factors

Planta
R LyckLutz Nover

Abstract

Similar to heat-stress transcription factors (HSFs) from non-plant sources, HSFA1 and HSFA2 from tomato (Lycopersicon esculentum Mill) contain two conserved clusters of basic amino acid residues (K/R1 and K/R2) which might serve as nuclear localization signal (NLS) motifs. Mutation of either one of them and functional testing of the corresponding proteins in a transient expression assay using tobacco (Nicotiana plumbaginifolia L:) protoplasts gave the following results. Whereas K/R1, positioned in all HSFs at the C-terminus of the DNA-binding domain, had no influence on nuclear import, the K/R1 mutants were impaired in their interaction with the DNA (band-shift assays). In contrast to this, mutants of the K/R2 motif, found 15-20 amino acid residues C-terminal of the oligomerization domain (HR-A/B region), had wild-type activity in DNA-binding but were defective in nuclear import. Thus, for the related tomato HSFA1 and HSFA2 the K/R2 cluster represents the only NLS motif, and in this function it cannot be replaced by K/R1.

Citations

Dec 31, 2004·Journal of Biosciences·Sanjeev Kumar BaniwalPascal von Koskull-Döring
May 26, 2010·Plant Cell Reports·Haibo XinMingfang Yi
Dec 18, 2004·Plant Molecular Biology·Eva Czarnecka-VernerWilliam B Gurley
Mar 6, 1999·Current Opinion in Plant Biology·A Nagatani
Nov 28, 2001·The Plant Journal : for Cell and Molecular Biology·L ZhangR Prändl
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·C. SchwechheimerM. W. Bevan
May 29, 2002·Proceedings of the National Academy of Sciences of the United States of America·Utako YamanouchiKyoji Yamada
Mar 29, 2008·Cell Stress & Chaperones·Masood SiddiqueKlaus-Dieter Scharf
Mar 26, 2013·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Eunsook ChungJai-Heon Lee
Oct 29, 2011·Biochimica Et Biophysica Acta·Klaus-Dieter ScharfLutz Nover
Dec 2, 2009·Biochimica Et Biophysica Acta·Donatella CanellaMichael F Thomashow
Apr 15, 2008·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Jingkang GuoJian Wang
Nov 15, 2007·The Plant Journal : for Cell and Molecular Biology·Franziska SchrammPascal von Koskull-Döring
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Jan 22, 2009·Plant, Cell & Environment·Joanna TrippKlaus-Dieter Scharf
Jul 23, 2008·The Plant Journal : for Cell and Molecular Biology·Christian WeberMarkus Fauth
Aug 26, 2016·The Plant Journal : for Cell and Molecular Biology·Sascha RöthEnrico Schleiff

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