Intracellular distribution of oxidized proteins and proteasome in HT22 cells during oxidative stress

Free Radical Biology & Medicine
Tobias JungTilman Grune

Abstract

The production of free radicals and the resulting oxidative damage of cellular structures are always connected with the formation of oxidized proteins. The 20S proteasome is responsible for recognition and degradation of oxidatively damaged proteins. No detailed studies on the intracellular distribution of oxidized proteins during oxidative stress and on the distribution of the proteasome have been performed until now. Therefore, we used immunocytochemical methods to measure protein carbonyls, a form of protein oxidation products, and proteasome distribution within cells. Both immunocytochemical methods of measurement are semiquantitative and the load of oxidized proteins is increased after various oxidative stresses explored, with the highest increase in the perinuclear region of the cell. Distribution of the proteasome and the total protein content revealed the highest concentration of both in the nucleus. No redistribution of the proteasome during oxidative stress occurs. The normalized ratio of protein carbonyls to protein content was formed, indicating the highest concentration of oxidized proteins in the cytosolic region near the cell membrane. By forming the protein oxidation-to-proteasome ratio it was concluded that the...Continue Reading

References

Jan 1, 1990·Methods in Enzymology·R L LevineE R Stadtman
Jan 1, 1984·Methods in Enzymology·H M Hassan
Jan 1, 1995·Free Radical Biology & Medicine·W Vogt
Jan 1, 1994·Methods in Enzymology·R L LevineE Shacter
Jun 28, 1996·The Journal of Biological Chemistry·T GruneK J Davies
Jan 1, 1997·Free Radical Biology & Medicine·H BussC C Winterbourn
Oct 8, 1997·The EMBO Journal·E A ReitsJ Trowsdale
Oct 23, 1997·Proceedings of the National Academy of Sciences of the United States of America·L J YanR S Sohal
Jun 6, 1998·The Journal of Biological Chemistry·T GruneK J Davies
Jun 20, 1998·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M A SmithG Perry
Jun 2, 1998·Drug Metabolism Reviews·E R Stadtman, B S Berlett
Sep 1, 1999·The International Journal of Biochemistry & Cell Biology·J M FaganI Sohar
Sep 18, 1999·Free Radical Biology & Medicine·O UllrichT Grune
Feb 23, 2000·Archives of Biochemistry and Biophysics·K MerkerT Grune
May 20, 2000·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·I PetropoulosB Friguet
Jun 23, 2000·Annals of the New York Academy of Sciences·E R Stadtman, R L Levine
Oct 21, 2000·Advances in Clinical Chemistry·J W Naskalski, G Bartosz
Dec 25, 2000·The Journal of Biological Chemistry·P LaschT Grune
Oct 5, 2001·Free Radical Biology & Medicine·O Ullrich, T Grune
Oct 20, 2001·Redox Report : Communications in Free Radical Research·W H Koppenol
Nov 16, 2001·Biogerontology·T Grune
Jan 10, 2002·The Journal of Biological Chemistry·Qunxing DingJeffrey N Keller
Mar 28, 2002·Physiological Reviews·Michael H Glickman, Aaron Ciechanover
Apr 2, 2002·Journal of Molecular Biology·Andrea LehmannCordula Enenkel
May 30, 2002·Biological Chemistry·Jana Mehlhase, Tilman Grune
Jun 25, 2002·Molecular Aspects of Medicine·Helen R GriffithsSiân B Astley
Jun 28, 2002·Free Radical Biology & Medicine·Pamela A SzwedaLuke I Szweda
Sep 5, 2002·Free Radical Biology & Medicine·Ulf T Brunk, Alexei Terman
Oct 4, 2002·Free Radical Biology & Medicine·Rachael A DunlopRoger T Dean
Oct 29, 2002·The Journal of Biological Chemistry·Reshma ShringarpureKelvin J A Davies
Dec 20, 2002·Journal of Structural Biology·Thomas Dino Rockel, Anna von Mikecz
Feb 18, 2003·Journal of Toxicology and Environmental Health. Part a·M Firoze KhanPaul J Boor
Aug 5, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Katrin MerkerTilman Grune
Aug 28, 2003·The EMBO Journal·Andreas FörsterChristopher P Hill

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Citations

Jun 28, 2011·Acta Neuropathologica·Victòria AyalaManuel Portero-Otin
Aug 20, 2009·The Journal of Biological Chemistry·Betul CatalgolTilman Grune
Mar 17, 2009·American Journal of Respiratory and Critical Care Medicine·Stephan Urs SixtJürgen Peters
Jan 23, 2008·Biological Chemistry·Nicolle Breusing, Tilman Grune
Jun 3, 2011·World Journal of Gastroenterology : WJG·Fawzia Bardag-Gorce
Feb 14, 2016·Free Radical Research·Alex J WadleySarah Aldred
Apr 30, 2009·International Journal of Radiation Biology·James M BrushKeisuke S Iwamoto
Jun 12, 2012·Free Radical Biology & Medicine·Marc KästleTilman Grune
Oct 28, 2010·Free Radical Biology & Medicine·Edina BakondiTilman Grune
Apr 21, 2010·Experimental Gerontology·Nicolle Breusing, Tilman Grune
Oct 27, 2009·Free Radical Biology & Medicine·Tobias JungTilman Grune
May 29, 2007·Experimental Gerontology·Nicolle BaderTilman Grune
Feb 27, 2007·Free Radical Biology & Medicine·Tobias JungTilman Grune
Jul 19, 2008·IUBMB Life·Tobias Jung, Tilman Grune
Jul 9, 2013·Mass Spectrometry Reviews·Maria FedorovaRalf Hoffmann
May 6, 2014·BioMed Research International·Sharifeh MehrabiFelix O Aikhionbare
Apr 7, 2015·Archives of Biochemistry and Biophysics·Valentina R GarbarinoRochelle Buffenstein
Jun 13, 2015·International Journal of Clinical Medicine·Sharifeh MehrabiFelix O Aikhionbare
Jul 15, 2016·Antioxidants & Redox Signaling·Annika HöhnTobias Jung
Apr 18, 2009·Molecular Aspects of Medicine·Tobias JungTilman Grune
Jan 13, 2009·Archives of Biochemistry and Biophysics·Tobias JungTilman Grune
Oct 14, 2017·The Journal of Physiology·Laura C D Pomatto, Kelvin J A Davies
Jul 28, 2009·Free Radical Research·Lucia RackovaTilman Grune
Jun 10, 2010·Free Radical Research·Cristini KleinMara S Benfato
Jan 17, 2019·Cell Biology and Toxicology·Graziano ColomboIsabella Dalle-Donne
Oct 25, 2017·Oxidative Medicine and Cellular Longevity·Antonio MichelucciSimona Boncompagni
Aug 2, 2017·Redox Biology·Ioanna KorovilaTobias Jung
Apr 28, 2021·Cell Chemical Biology·Fulya TürkerSeth S Margolis
May 16, 2021·Journal of Cosmetic Dermatology·Changhui ChoJaesung Hwang
Mar 17, 2007·Archives of Biochemistry and Biophysics·Tobias JungTilman Grune

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