Intracellular distribution of poly(ADP-ribose) synthetase in rat spermatogenic cells

Experimental Cell Research
I I ConchaL O Burzio

Abstract

The highest activity of poly(ADP-ribose) synthetase was found in the testis among several rat tissues tested. Subcellular fractionation of the testis demonstrated that the synthetase was localized primarily in the nucleus and partially in the microsomal-ribosomal fraction. This result was confirmed by immunocytochemical staining with the enzyme-specific antibody. The synthetase was localized in the nuclei of interstitial cells, Sertoli cells, spermatogonia, and spermatocytes. In addition, round spermatids showed a granular staining in the cytoplasm, which was comparable in intensity with that in the nucleus. The cytoplasmic synthetase had a molecular weight of 115,000 and synthesized oligomers of ADP-ribose on itself (automodification). The synthetase activity in the isolated cytoplasmic fraction was stimulated about threefold by the addition of DNA and depressed by treatment with DNase I, suggesting the presence of endogenous activator DNA. A candidate DNA for such an activator was isolated from the microsomal-ribosomal fraction, and identified tentatively as mitochondrial DNA on the basis of its size and restriction fragment patterns.

References

Mar 1, 1976·Archives of Biochemistry and Biophysics·L O BurzioS S Koide
Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·T R ButtM E Smulson
Nov 1, 1975·Archives of Biochemistry and Biophysics·J H RobertsM Smulson
Oct 24, 1977·Biochemical and Biophysical Research Communications·K YoshiharaH Ohgushi
Nov 1, 1977·Experimental Cell Research·S R GrimesL S Hnilica
Jan 1, 1985·Annual Review of Biochemistry·K Ueda, O Hayaishi
Dec 17, 1985·Biochemical and Biophysical Research Communications·H ThomassinP Mandel
Jul 1, 1984·The Biochemical Journal·M R Faraone MennellaR Jones
Nov 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·Y OhashiO Niwa
Aug 1, 1983·Analytical Biochemistry·K Hancock, V C Tsang
Feb 7, 1980·Nature·B W DurkaczS Shall
Nov 17, 1980·Biochemical and Biophysical Research Communications·K Ikai, K Ueda
Nov 16, 1981·Biochemical and Biophysical Research Communications·L O BurzioR Cornejo
May 1, 1982·Archives of Biochemistry and Biophysics·M AgemoriY Shizuta
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·D DiMaioT Maniatis
Nov 1, 1983·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·K Ikai, K Ueda
Nov 2, 1983·European Journal of Biochemistry·J Jongstra-BilenP Mandel
Jun 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·G G PoirierP Mandel
Mar 1, 1957·The American Journal of Anatomy·Y CLERMONT, B PEREY

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Citations

Apr 20, 2005·Chromosoma·Mirella L Meyer-FiccaRalph G Meyer
Apr 1, 2009·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Michèle RouleauGuy G Poirier
Apr 12, 2002·The Journal of Biological Chemistry·Valérie SchreiberGilbert de Murcia
May 22, 2020·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Maria Tedim FerreiraGuy G Poirier

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