Intracellular distributions of essential elements in cardiomyocytes

Journal of Structural Biology
Bradley M PalmerMartin M Lewinter

Abstract

We describe the intracellular distributions of nine essential elements (P, S, Cl, K, Ca, Mn, Fe, Cu, and Zn) found in cardiomyocytes imaged using synchrotron X-ray induced fluorescence. Cardiomyocytes were isolated from rat hearts, flash frozen on Si(3)N(4) windows, freeze-dried, and imaged with approximately 300 nm spatial resolution. Distinct longitudinal patterns in cardiomyocytes were most apparent for the elements Fe and Cu, which clearly colocalized. Transverse striations were apparent for P, S, Fe, and Zn, while those for Zn were consistently the most prominent ( approximately 10(-3)M) and appeared with a periodicity in the range 1.63-1.75 microm, the expected length of a sarcomere. Transverse striations for high concentrations of P, Fe, and Zn and low concentrations of S colocalized and coincided with the I-band of the intact cardiomyocyte. Fluorescence microscopy using FluoZin-3 in intact cardiomyocytes suggests that Zn(2+) influx is through sarcolemmal calcium channels and that significant stores of intracellular Zn(2+) may be released quickly (<1s) into the cytosol. These data collectively suggest that Zn(2+) is buffered by structures associated near the T-tubules and/or in the sarcoplasmic reticulum and is found in ...Continue Reading

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Citations

Jan 5, 2008·Photochemistry and Photobiology·Yu-Ying HeColin F Chignell
Oct 31, 2012·Cellular and Molecular Life Sciences : CMLS·Marta GaburjakovaMuthu Periasamy
Jun 4, 2008·AIDS·Neil H ShearElizabeth J Phillips
Jan 27, 2011·The Biochemical Journal·Naresh C BalMuthu Periasamy
Sep 29, 2006·Journal of Cellular Biochemistry·Tatjana PauneskuGayle Woloschak
Oct 8, 2014·Journal of Cardiovascular Pharmacology·Nephertiti EfeovbokhanKarl T Weber
Jul 13, 2014·American Journal of Physiology. Heart and Circulatory Physiology·Yuan WangBradley M Palmer
Jul 3, 2013·American Journal of Physiology. Heart and Circulatory Physiology·Ting YiBradley M Palmer
Jun 24, 2020·Cell Calcium·Qian Wang, Marek Michalak

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