Intracellular domain of nicotinic acetylcholine receptor: the importance of being unfolded

Journal of Neurochemistry
Viktoria KukhtinaFerdinand Hucho

Abstract

Bioinformatics methods with subsequent verification by experimental data were applied to the structural investigation of the intracellular loop of the delta-subunit of the nicotinic acetylcholine receptor (nAChR). Three complementary methods were used: prediction of secondary structure elements, prediction of ordered/disordered protein regions and prediction of short functional binding motifs. The output of five different algorithms was used for the secondary structure construction. Most of the intracellular domain is predicted to be unfolded. The predictions correlate well with the experimental data of limited proteolysis and NMR performed on the mostly monomeric fraction of heterologously expressed Torpedo intracellular domain protein. Twelve functional binding motifs within the disordered regions of the nAChR intracellular domain are predicted. Identification of proteins that interact with the intracellular domain will provide a better understanding of protein-protein interactions involved in nAChR assembly, trafficking and clustering.

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Citations

Apr 2, 2011·The Journal of Biological Chemistry·Matthew R LiveseyJohn A Peters
Aug 17, 2011·The Journal of Biological Chemistry·Raman GoyalMichaela Jansen
Sep 14, 2013·The Journal of Biological Chemistry·Jane E CarlandJeremy J Lambert
Jan 8, 2009·Physiological Reviews·Edson X AlbuquerqueScott W Rogers
May 30, 2008·Frontiers in Bioscience : a Journal and Virtual Library·Gregg B Wells
Jun 24, 2014·Journal of Molecular Graphics & Modelling·William J BelfieldP-L Chau
Mar 26, 2009·IUBMB Life·Marios ZouridakisSocrates J Tzartos
Jul 27, 2007·The FEBS Journal·Dimitra KalamidaSocrates J Tzartos
Nov 29, 2013·British Journal of Pharmacology·J L KozuskaS M J Dunn
Dec 13, 2006·Biophysical Journal·Predrag RadivojacA Keith Dunker
Feb 6, 2018·Chembiochem : a European Journal of Chemical Biology·Ferenc Zsila
Jun 27, 2018·Journal of Evolutionary Biology·Guillermo RealesMaria Cátira Bortolini
Oct 8, 2020·Journal of Cosmetic Dermatology·Pengfei SunBo Pan

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