Intracellular dynamics of polydnavirus innexin homologues

Insect Molecular Biology
Daniel K HasegawaMatthew W Turnbull

Abstract

Polydnaviruses associated with ichneumonid parasitoid wasps (Ichnoviruses) encode large numbers of genes, often in multigene families. The Ichnovirus Vinnexin gene family, which is expressed in parasitized lepidopteran larvae, encodes homologues of Innexins, the structural components of insect gap junctions. Here, we have examined intracellular behaviours of the Campoletis sonorensis Ichnovirus (CsIV) Vinnexins, alone and in combination with a host Innexin orthologue, Innexin2 (Inx2). QRT-PCR verified that transcription of CsIV vinnexins occurs contemporaneously with inx2, implying co-occurrence of Vinnexin and Inx2 proteins. Confocal microscopy demonstrated that epitope-tagged VinnexinG (VnxG) and VinnexinQ2 (VnxQ2) exhibit similar subcellular localization as Spodoptera frugiperda Inx2 (Sf-Inx2). Surface biotinylation assays verified that all three proteins localize to the cell surface, and cytochalasin B and nocodazole that they rely on actin and microtubule cytoskeletal networks for localization. Immunomicroscopy following co-transfection of constructs indicates extensive co-localization of Vinnexins with each other and Sf-Inx2, and live-cell imaging of mCherry-labelled Inx2 supports that Vinnexins may affect Sf-Inx2 distrib...Continue Reading

References

Mar 20, 1999·The Journal of Biological Chemistry·C H GeorgeW H Evans
Apr 19, 2002·Mechanisms of Development·Lucy A StebbingsJane A Davies
Aug 1, 2002·Proceedings of the National Academy of Sciences of the United States of America·Undine LaufMatthias M Falk
Sep 17, 2003·Insect Molecular Biology·M D Lavine, M R Strand
Mar 30, 2004·Molecular Biology of the Cell·Reinhard BauerMichael Hoch
Oct 9, 2004·Science·Eric EspagneJean Michel Drezen
Jul 12, 2005·Current Biology : CB·Matthew W TurnbullPauline Phelan
Jan 27, 2006·Molecular Biology of the Cell·Corinna LehmannMichael Hoch
Feb 20, 2007·Virology·Kohjiro TanakaBruce A Webb
Jan 22, 2008·The Journal of Experimental Biology·Xing-He WengKlaus W Beyenbach
Jan 7, 2009·Insect Biochemistry and Molecular Biology·Sarit AnavaAmir Ayali
Feb 14, 2009·Science·Annie BézierJean-Michel Drezen
May 6, 2009·Molecular Biotechnology·Sun Mee HongTakahiro Kusakabe
Jan 21, 2010·The Journal of Biological Chemistry·Ruchi Bhalla-GehiDale W Laird
Jun 5, 2010·PLoS Pathogens·Anne-Nathalie VolkoffJean-Michel Drezen
Apr 28, 2011·Journal of Insect Science·Kaijun Luo, Matthew W Turnbull
Aug 5, 2011·Journal of Virology·N K MarzianoM W Turnbull
Jul 3, 2013·Current Opinion in Virology·Michael R Strand, Gaelen R Burke
Mar 19, 2014·FEBS Letters·Daniel K Hasegawa, Matthew W Turnbull
Jul 19, 2014·The Journal of Biological Chemistry·Michelle W M LiErol Fikrig
May 6, 2015·Archives of Insect Biochemistry and Physiology·Zunyu PangKaijun Luo

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.