Intracellular Dynamics of the Ubiquitin-Proteasome-System

F1000Research
Maisha Chowdhury, C Enenkel

Abstract

The ubiquitin-proteasome system is the major degradation pathway for short-lived proteins in eukaryotic cells. Targets of the ubiquitin-proteasome-system are proteins regulating a broad range of cellular processes including cell cycle progression, gene expression, the quality control of proteostasis and the response to geno- and proteotoxic stress. Prior to degradation, the proteasomal substrate is marked with a poly-ubiquitin chain. The key protease of the ubiquitin system is the proteasome. In dividing cells, proteasomes exist as holo-enzymes composed of regulatory and core particles. The regulatory complex confers ubiquitin-recognition and ATP dependence on proteasomal protein degradation. The catalytic sites are located in the proteasome core particle. Proteasome holo-enzymes are predominantly nuclear suggesting a major requirement for proteasomal proteolysis in the nucleus. In cell cycle arrested mammalian or quiescent yeast cells, proteasomes deplete from the nucleus and accumulate in granules at the nuclear envelope (NE) / endoplasmic reticulum (ER) membranes. In prolonged quiescence, proteasome granules drop off the NE / ER membranes and migrate as stable organelles throughout the cytoplasm, as thoroughly investigated i...Continue Reading

References

Oct 1, 1990·FEBS Letters·K TanakaA Ichihara
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·E EytanA Hershko
Jan 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·A AmsterdamW Baumeister
Jan 1, 1996·Annual Review of Biochemistry·O CouxA L Goldberg
May 5, 1997·The Journal of Cell Biology·H B McDonald, B Byers
May 30, 1997·Cell·M P RoutJ D Aitchison
Oct 8, 1997·The EMBO Journal·E A ReitsJ Trowsdale
Jun 20, 1998·Molecular and Cellular Biology·M H GlickmanD Finley
Aug 8, 1998·Trends in Cell Biology·R W WozniakJ D Aitchison
Oct 6, 1998·Annual Review of Biochemistry·A Hershko, A Ciechanover
Oct 6, 1998·Annual Review of Biochemistry·R Y Tsien
Nov 21, 1998·The EMBO Journal·C R WilkinsonC Gordon
Jul 27, 1999·The Journal of Biological Chemistry·S J RussellS A Johnston
Dec 28, 1999·Trends in Cell Biology·M Kirschner
Dec 28, 1999·Annual Review of Cell and Developmental Biology·D Görlich, U Kutay
Jan 5, 2000·The EMBO Journal·J S ThrowerC M Pickart
Mar 16, 2000·The Laryngoscope·D A LeopoldG Kobal
Nov 4, 2000·Nature Structural Biology·M GrollD Finley
Feb 21, 2002·Molecular Biology of the Cell·Nelly Panté, Michael Kann
Apr 2, 2002·Journal of Molecular Biology·Andrea LehmannCordula Enenkel
Dec 14, 2002·Science·Chang-Wei LiuPhilip J Thomas
Apr 4, 2003·The International Journal of Biochemistry & Cell Biology·Cezary Wójcik, George N DeMartino
Apr 4, 2003·The International Journal of Biochemistry & Cell Biology·Graciela FuertesErwin Knecht
May 14, 2003·Trends in Cell Biology·Herbert Tschochner, Ed Hurt
Jul 5, 2003·Current Biology : CB·Monika BajorekMichael H Glickman
Oct 17, 2003·Nature·Won-Ki HuhErin K O'Shea
Jan 18, 2005·Trends in Cell Biology·Martin Rechsteiner, Christopher P Hill
Dec 13, 2005·Methods in Enzymology·Tom A M Groothuis, Eric A J Reits
Dec 24, 2005·Science·Ramunas M Vabulas, F Ulrich Hartl
Mar 28, 2006·Cell·J Wade Harper, Brenda A Schulman
May 12, 2006·Journal of Cell Science·Anna von Mikecz

❮ Previous
Next ❯

Citations

Jun 22, 2018·Annual Review of Biochemistry·Charisma EnamRichard G Gardner
Sep 30, 2016·Critical Reviews in Biochemistry and Molecular Biology·Ravikiran S YedidiCordula Enenkel
Apr 7, 2018·ELife·Richard Scott Marshall, Richard David Vierstra
Jan 31, 2020·Frontiers in Molecular Biosciences·Ofri Karmon, Shay Ben Aroya
Apr 4, 2020·Scientific Reports·Yuki KitoKeiichi I Nakayama
Jan 14, 2016·Nucleus·Mirta Boban, Roland Foisner
Aug 28, 2020·Cancers·Claudio Brancolini, Luca Iuliano
Oct 24, 2018·Molecular and Cellular Biology·Takuya TomitaShigeo Murata
Jan 8, 2019·Biochemical Pharmacology·Flavia RadognaMarc Diederich

❮ Previous
Next ❯

Methods Mentioned

BETA
X-ray
fluorescence microscopy
fluorescence recovery after photobleaching
nucleotide exchange
electron microscopy
fluorescence correlation spectroscopy

Related Concepts

Related Feeds

Cell Cycle Control & Proteolysis

Key regulators of cell cycle, including cyclins, cyclin dependent kinase inhibitors, DNA replication factors, are controlled by proteolysis. Discover the latest research on cell cycle control and proteolysis.