Intracellular Environment and agr System Affect Colony Size Heterogeneity of Staphylococcus aureus

Frontiers in Microbiology
Nicola HäffnerAnnelies S Zinkernagel

Abstract

Staphylococcus aureus causes chronic and relapsing infections, which may be difficult to treat. So-called small colony variants (SCVs) have been associated with chronic infections and their occurrence has been shown to increase under antibiotic pressure, low pH and intracellular localization. In clinics, S. aureus isolated from invasive infections often show a dysfunction in the accessory gene regulator (agr), a major virulence regulatory system in S. aureus. To assess whether intracellular environment and agr function influence SCV formation, an infection model was established using lung epithelial cells and skin fibroblasts. This allowed analyzing intracellular survival and localization of a panel of S. aureus wild type strains and their isogenic agr knock out mutants as well as a natural dysfunctional agr strain by confocal laser scanning microscopy (CLSM). Furthermore, bacterial colonies were quantified after 1, 3, and 5 days of intracellular survival by time-lapse analysis to determine kinetics of colony appearance and SCV formation. Here, we show that S. aureus strains with an agr knock out predominantly resided in a neutral environment, whereas wild type strains and an agr complemented strain resided in an acidic environ...Continue Reading

References

Jan 1, 1987·Advances in Experimental Medicine and Biology·F R Maxfield, D J Yamashiro
Jan 1, 1971·Acta Pathologica Et Microbiologica Scandinavica. Section B: Microbiology and Immunology·S Arvidson, T Holme
Sep 1, 1971·Journal of General Microbiology·J BohácekT Martinec
Aug 26, 1998·The New England Journal of Medicine·F D Lowy
Jan 18, 2002·Infection and Immunity·Jon S BlevinsMark S Smeltzer
Jul 3, 2002·Infectious Disease Clinics of North America·Cathy A Petti, Vance G Fowler
Dec 4, 2004·Journal of Bacteriology·Brian WeinrickRichard P Novick
Jun 3, 2005·The Journal of Bone and Joint Surgery. American Volume·Michael J BosseWarren K Ramp
Aug 19, 2005·The Journal of Infectious Diseases·Sophie ClementJean-Silvain Lacroix
Mar 17, 2006·Nature Reviews. Microbiology·Richard A ProctorGeorg Peters
Dec 1, 2006·The Journal of Biological Chemistry·Annabelle SchnaithOleg Krut
May 22, 2007·Journal of Microbiological Methods·Thanh T Luong, Chia Y Lee
Aug 30, 2008·The Journal of Infectious Diseases·Bo ShopsinRichard P Novick
Jan 24, 2009·Trends in Microbiology·Parham Sendi, Richard A Proctor
Jul 18, 2009·The Journal of Antimicrobial Chemotherapy·Fred C Tenover, Richard V Goering
Feb 6, 2010·The Journal of Infectious Diseases·Matthias GrundmeierBettina Löffler
Dec 22, 2010·Antimicrobial Agents and Chemotherapy·Marin L SchweizerEli N Perencevich
Sep 1, 2011·The EMBO Journal·Jatta Huotari, Ari Helenius
Oct 26, 2011·Proceedings of the National Academy of Sciences of the United States of America·Frank R DeLeoBarry N Kreiswirth
Feb 3, 2012·The New England Journal of Medicine·Daniel H LibratyBrenda Torres
Aug 25, 2012·Frontiers in Cellular and Infection Microbiology·Martin Fraunholz, Bhanu Sinha
Jan 1, 2013·Journal of Microbiological Methods·Kati Seidl, Annelies S Zinkernagel
Dec 25, 2013·International Journal of Medical Microbiology : IJMM·Bettina LöfflerGeorg Peters
Jul 10, 2016·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M StrobelS Niemann
Dec 14, 2016·International Journal of Medical Microbiology : IJMM·Kati SeidlAnnelies S Zinkernagel
May 23, 2017·Nature Reviews. Microbiology·Robert A FisherSophie Helaine
Aug 5, 2017·Frontiers in Microbiology·Guillaume RollinMathieu Coureuil
Aug 1, 2018·Infection and Immunity·Deena R AltmanHarm van Bakel
Oct 6, 2018·Nature Communications·Clément VulinAnnelies S Zinkernagel

❮ Previous
Next ❯

Citations

Feb 8, 2021·Cellular Microbiology·Ye Mon SoeAbderrahman Hachani
Mar 2, 2021·Frontiers in Immunology·Brittney D Gimza, James E Cassat
Apr 4, 2021·Toxins·Cassandra PougetJean-Philippe Lavigne
Jun 17, 2021·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Helen L BrownPhil Stephens
Oct 13, 2021·MSystems·Charlotte OriolAstrid Rouillon

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
Assay

Software Mentioned

Imaris
ggplot
CLC Genomic Workbench (
ggbeeswarm
R Studio
R
Huygens Professional
FlowJo
pylon
ColTapp

Related Concepts

Related Feeds

Antimalarial Agents (ASM)

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

Antimalarial Agents

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.