Intracellular fate of octaarginine-modified liposomes in polarized MDCK cells

International Journal of Pharmaceutics
Takahiro FujiwaraH Harashima

Abstract

Octaarginine (R8)-modified liposomes have been used to deliver therapeutic substances into cells owing to the efficient cellular uptake via macropinocytosis. Recent analyses revealed that R8-modified liposomes are mainly taken up via macropinocytosis, and escape from endosomes efficiently to avoid lysosomal degradation in non-polarized NIH-3T3 cells. In the present study, we evaluated the intracellular fate of R8-modified liposomes in polarized MDCK cells, comparing their trafficking with that of conventional cationic liposomes by confocal laser scanning microscopy (CLSM). In contrast to what occurs in NIH-3T3 cells, R8-modified liposomes are internalized by MDCK cells equally well via clathrin-mediated endocytosis and macropinocytosis. The most salient characteristic in subsequent intracellular trafficking in MDCK cells is that R8-modified liposomes become trapped in the endosomal compartment and subsequently, a portion of them colocalizes with the Golgi apparatus. Similar colocalization with the Golgi apparatus was observed for octalysine (K8)-modified liposomes. In contrast, cationic liposomes were found to colocalize predominantly with lysosomes stained with lysotracker. Collectively, in polarized MDCK cells, cationic pepti...Continue Reading

Citations

Jan 26, 2013·International Journal of Pharmaceutics·Qing LvJian-Qing Gao
Dec 15, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Kasper Bendix Johnsen, Torben Moos
Jul 6, 2010·Mitochondrion·Lucia BiasuttoJiri Neuzil
Sep 29, 2011·Biomaterials·Koki KamiyaKazunari Akiyoshi
Nov 26, 2010·International Journal of Pharmaceutics·Tomohiro IzumisawaYoshie Maitani
Apr 21, 2016·Drug Discovery Today·Weiwei FanYong Gan
Nov 28, 2012·Advanced Healthcare Materials·Juan GalloSoledad Penadés
Jan 21, 2014·Advanced Healthcare Materials·Paolo VerderioDavide Prosperi
Jul 23, 2013·Macromolecular Bioscience·Dahai YuChangyou Gao
Jul 24, 2020·Journal of Drug Targeting·Zhijing HeGuangxi Zhai

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