Intracellular fluorescent temperature probe based on triarylboron substituted poly N-isopropylacrylamide and energy transfer

Analytical Chemistry
Jun LiuGuoqiang Yang

Abstract

A novel hydrophilic fluorescence temperature probe (PNDP) based on polarity-sensitive triarylboron compound (DPTB) and PNIPAM is designed and synthesized. In order to overcome the shortcomings of the single-intensity-based sensing mechanism and obtain more robust signals, ratiometric readout is achieved by designing an efficient FRET system (PNDP-NR) between DPTB and Nile Red (NR). PNDP-NR possesses some excellent features, including wide temperature range, good linear relationship, high temperature resolution, excellent reversibility, and stability. Within a sensing temperature range of 30-55 °C, the fluorescence color of PNDP-NR experiences significant change from red to green-blue. PNDP-NR is also introduced into NIH/3T3 cells to sense the temperature at the single-cell level. It gave excellent photostability and low cytotoxicity in vivo.

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Citations

Apr 22, 2017·Chemistry : a European Journal·Sven OttoKatja Heinze
Mar 14, 2017·Chemical Communications : Chem Comm·Qiyun FangGuoqiang Yang
Mar 8, 2017·Chemical Communications : Chem Comm·Sheng-Yong LiCui-Hua Zhao
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Oct 8, 2016·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Seiichi UchiyamaChie Gota
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Mar 15, 2019·Biomicrofluidics·Fan YangShengyong Xu
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Dec 28, 2016·ACS Applied Materials & Interfaces·Junjie CuiSoo Young Park
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