Intracellular folding of the Tetrahymena group I intron depends on exon sequence and promoter choice

RNA
Sujatha Koduvayur, S A Woodson

Abstract

The Tetrahymena group I intron splices 20 to 50 times faster in Tetrahymena than in vitro, implying that the intron rapidly adopts its active conformation in the cell. The importance of cotranscriptional folding and the contribution of the rRNA exons to the stability of the active pre-RNA structure were investigated by comparing the activity of minimal pre-RNAs expressed in Escherichia coli. Pre-RNAs containing exons derived from E. coli 23 S rRNA were three to four times more active than the wild-type Tetrahymena pre-RNA. E. coli transcripts of the chimeric E. coli pre-RNA were two to eight times more active than were T7 transcripts. However, the effect of cotranscriptional folding depends on exon sequences. Unexpectedly, the unspliced pre-RNA decays more slowly than predicted from the rate of splicing. This observation is best explained by partitioning of transcripts into active and inactive pools. We propose that the active pool splices within a few seconds, whereas the inactive pool is degraded without appreciable splicing.

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Citations

Apr 18, 2007·Proceedings of the National Academy of Sciences of the United States of America·Alain LaederachMichael Brenowitz
Feb 18, 2010·PLoS Biology·Elisabeth M MahenMartha J Fedor
Apr 30, 2013·Nucleic Acids Research·Jing Yun A ZhuIrmtraud M Meyer
Oct 18, 2013·RNA·Daniel LaiIrmtraud M Meyer
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Sep 4, 2007·Journal of Molecular Biology·Richard A LeaseSarah A Woodson
Jan 27, 2015·Nucleic Acids Research·Meredith CorleyAlain Laederach
Nov 25, 2014·Nucleic Acids Research·Steven W SowaLydia M Contreras
Dec 30, 2014·Nucleic Acids Research·Hui-Ting LeeSarah A Woodson
Jul 28, 2016·Journal of Molecular Biology·Brant GraciaRick Russell
Feb 1, 2018·Wiley Interdisciplinary Reviews. RNA·Chang Ho LeeSeong-Wook Lee
Nov 4, 2010·RNA Biology·Sarah A Woodson
Nov 4, 2010·RNA Biology·Georgeta Zemora, Christina Waldsich
Jun 29, 2021·Trends in Biochemical Sciences·Margaret L Rodgers, Sarah A Woodson
Aug 1, 2018·Journal of the American Chemical Society·Boyang HuaTaekjip Ha

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