PMID: 6987896Feb 1, 1980Paper

Intracellular glucose concentration in small and large rat adipose cells

The American Journal of Physiology
J E FoleyL B Salans

Abstract

Intracellular free glucose concentrations have been estimated in small and large isolated epididymal adipose cells prepared from young lean and older obese rats using glucose-induced steady-state tracer 3-O-methylglucose countertransport. Steady-state 3-O-methylglucose uptake was measured in the presence of 2--50 mM glucose or sucrose in the absence or presence of 100 microU insulin/ml. The ratio of the uptake of 3-O-methylglucose in the presence of glucose to that in the presence of sucrose at each sugar concentration was then utilized to estimate the corresponding intracellular glucose concentration. At all three extracellular glucose concentrations tested in the absence of insulin, intracellular concentrations of glucose are greater in the large cells than in the small cells. In the presence of 2 mM glucose and insulin, on the other hand, intracellular glucose concentrations of 2 mM are attained, regardless of cell size. These results suggest that transport may not be the rate-limiting step for glucose metabolism at physiologic glucose concentrations either in the enlarged adipose cell in the absence or presence of insulin or in the small adipose cell in the presence of maximally stimulating concentrations of insulin.

References

Jun 1, 1976·The Journal of Clinical Investigation·M P Czech
Mar 26, 1976·Molecular and Cellular Biochemistry·M P Czech
Dec 11, 1974·Biochemical and Biophysical Research Communications·J N Livingston, D H Lockwood
Aug 18, 1972·Science·J N LivingstonD H Lockwood

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Citations

Jan 31, 2003·American Journal of Physiology. Endocrinology and Metabolism·Hilary Ann PetersenAmy E Halseth
May 15, 2001·American Journal of Physiology. Endocrinology and Metabolism·A E HalsethD H Wasserman
Jan 9, 2013·Journal of Diabetes Science and Technology·Jithesh V VeetilKaiming Ye
Oct 29, 2000·American Journal of Physiology. Endocrinology and Metabolism·A E HalsethD H Wasserman
Apr 10, 2003·American Journal of Physiology. Endocrinology and Metabolism·Werner RegittnigThomas R Pieber

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