Intracellular hemolysin-producing Listeria monocytogenes strains inhibit macrophage-mediated antigen processing.

Infection and Immunity
C W CluffH K Ziegler

Abstract

We found that virulent hemolysin-producing (Hly+) Listeria monocytogenes strains inhibit antigen processing and presentation when added to macrophages in vitro. A virulent Hly- bacteria caused little or no inhibition. Live Hly+ bacteria inhibited presentation of both heat-killed L. monocytogenes and ovalbumin. Several observations indicate that hemolysin produced by intracellular bacteria was responsible for the inhibition. First, inhibition was observed even when extracellular bacteria were removed after a brief 10-min bacterial uptake period. Second, inhibition was not prevented by the addition of cholesterol, a substance which inactivates soluble hemolysin. Third, only very high concentrations of soluble hemolysin were inhibitory. Under conditions which inhibit antigen presentation (10(5) per well), macrophages retained normal levels of Ia, maintained normal morphology, and were not permeable when assayed by chromium release. The uptake and catabolism of 35S-labeled live bacteria by macrophages were similar for both Hyl+ and Hly- bacteria. Only a small decrease in uptake and catabolism of surface-iodinated heat-killed L. monocytogenes by macrophages pretreated with inhibitory numbers of live Hly+ bacteria was observed. Addit...Continue Reading

References

Jun 1, 1977·Journal of Clinical Microbiology·R D Groves, H J Welshimer
May 1, 1990·The Journal of Experimental Medicine·P E Jensen
Apr 1, 1988·Journal of Bacteriology·R A Welch, S Pellett
Apr 1, 1988·The Journal of Experimental Medicine·D A PortnoyD J Hinrichs
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·P Cresswell
Jul 1, 1986·The Journal of Experimental Medicine·S H KaufmannG De Libero
Nov 1, 1987·Journal of Clinical Microbiology·L PineG B Malcolm
Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·N A Buchmeier, R D Schreiber
Jun 1, 1966·Bacteriological Reviews·M L Gray, A H Killinger
Nov 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·H Z StreicherJ A Berzofsky
Oct 1, 1984·European Journal of Immunology·A F KiderlenM L Lohmann-Matthes
Jan 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·H K Ziegler, E R Unanue
Oct 1, 1963·The Biochemical Journal·F C GREENWOODJ S GLOVER
Aug 1, 1964·Journal of Bacteriology·E M JENKINSE W ADAMS

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Citations

Jan 1, 1991·Critical Reviews in Microbiology·V Braun, T Focareta
Jun 7, 1996·The Journal of Biological Chemistry·C Alvarez-DominguezP D Stahl
Feb 13, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·G GeginatH Hof
Oct 25, 1996·Annals of the New York Academy of Sciences·F D QuinnC H King
Jan 1, 1997·Critical Reviews in Microbiology·A K Bhunia
Jul 23, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mojca SkoberneGernot Geginat
Mar 19, 2002·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Paul Webster
Apr 1, 2000·Microbes and Infection·W P Maksymowych, K P Kane
Jul 1, 1995·Clinical and Diagnostic Laboratory Immunology·M LouieK Sperber
Oct 1, 1992·Infection and Immunity·R A GarduñoW W Kay

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