Intracellular hydrolases of Aspergillus parasiticus and Aspergillus flavus

Biochemical and Biophysical Research Communications
A SharmaV Ninjoor

Abstract

When the distribution profile of hydrolases in mycelial homogenates and culture filtrates of A. parasiticus and A. flavus was examined, six hydrolytic enzymes viz. N-acetyl-beta-glucosaminidase, aryl sulfatase, alkaline proteinase, cathepsin B, cathepsin D and aminopeptidase were detected in homogenate. The culture filtrates were devoid of any activity of these enzymes. The enzyme levels varied with the stage of incubation. The most abundant fungal exopeptidase showing preference for basic amino acid naphthylamides seems to be an aminopeptidase B. Incorporation of CEPA, an ethylene generating compound, stimulated the amino peptidase activity in the mycelium but inhibited the enzyme in vitro. The enzyme was also inhibited by different aflatoxins to varying degree. While aminopeptidase B was located intracellularly, a non-dialysable, heat-stable inhibitor of the enzyme was found to be secreted in the culture filtrate. This peptide inhibitor was however ineffective on the other enzymes.

References

Nov 1, 1976·Archives of Biochemistry and Biophysics·H SudaH Umezawa
Jun 1, 1964·Biochemical and Biophysical Research Communications·J K McDonaldS Ellis
Dec 1, 1984·Biochemical Society Transactions·A J Barrett

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Citations

Aug 2, 2003·International Journal of Food Microbiology·Tomás BolumarFidel Toldrá
Jul 20, 1999·Annual Review of Biophysics and Biomolecular Structure·M E McGrath
Feb 5, 1997·Chemical Reviews·Hans-Hartwig Otto, Tanja Schirmeister

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Aspergillosis (ASM)

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.

Aspergillosis

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.

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