Intracellular inorganic carbon exists as protein carbamate in photosynthesizing Euglena gracilis z

Biochemical and Biophysical Research Communications
A YokotaS Kitaoka

Abstract

The form of inorganic carbon accumulated in Euglena gracilis cells was determined. Euglena cell protein bound 0.96 nmol CO2/mg protein. The binding of CO2 was by the formation of protein carbamate as indicated by acid lability of the protein-CO2 complex, stimulation of complex formation by high ionic strength and the carbamate resonance in 13C-NMR spectrum. The protein carbamate could also be isolated from photosynthesizing Euglena. The formation of the carbamate required light energy fixed photosynthetically.

References

Jan 1, 1981·Methods in Enzymology·M Perrella, L Rossi-Bernardi
Dec 14, 1934·The Journal of Physiology·J K Ferguson, F J Roughton

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