Intracellular, interstitial and plasma spaces in the rat myocardium in vivo

Journal of Molecular and Cellular Cardiology
G P Dobson, J H Cieslar

Abstract

The aim of this study was to determine tissue water spaces in rat heart in vivo using [14C]-inulin or D-[14C]-mannitol as extracellular markers, and 2,3DPG as whole blood tissue marker. Tracer was injected into the jugular vein of anaesthetised male Sprague-Dawley rats (60 mg/kg) and allowed to equilibrate for 30 min. A blood sample was then taken and the heart was freeze-clamped at liquid N2 temperatures within 5 s of opening the chest cavity. Extracellular space was calculated by dividing the total tissue counts (d/min/g) by plasma counts (d/min/g). The extracellular inulin and mannitol spaces were 0.212+/-0.010 and 0.236+/-0.012 ml/g wet weight tissue, respectively (n=5). Expressed as a percentage of total tissue water (0.79+/-0.012 ml/g wet weight tissue, n=9), the intracellular space was found to be 73.1 (inulin) and 70.1% (mannitol). The interstitial space was calculated by subtracting the counts in tissue due to whole blood from total tissue counts and dividing by plasma counts. The interstitial space was 19.4 and 21. 9%; and the plasma space was 7.5 and 8.0% for the inulin and mannitol methods, respectively. We conclude that (i) there was no significant differences in the inulin or mannitol spaces in the in vivo heart (...Continue Reading

Citations

Apr 27, 2001·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·T Q DuongJ J Neil
Sep 14, 2006·Pharmaceutical Research·Pakawadee SermsappasukMichael Weiss
Mar 6, 2004·The Journal of Thoracic and Cardiovascular Surgery·Geoffrey P Dobson, Michael W Jones
Aug 2, 2003·Clinical and Experimental Pharmacology & Physiology·Geoffrey P Dobson
Jun 13, 2015·Journal of Cardiovascular Magnetic Resonance : Official Journal of the Society for Cardiovascular Magnetic Resonance·Christophe de Meester de RavensteinBernhard L Gerber
Apr 1, 2006·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Pakawadee SermsappasukMichael Weiss
Feb 2, 2017·Journal of Cardiovascular Magnetic Resonance : Official Journal of the Society for Cardiovascular Magnetic Resonance·Seiko IdeLars Grosse-Wortmann
Jul 20, 2002·The Journal of Pharmacology and Experimental Therapeutics·Wonku Kang, Michael Weiss
Aug 16, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Christian BollensdorffKlaus Benndorf
Oct 4, 2000·Physiological Reviews·O M Sejersted, G Sjøgaard
Jul 17, 2001·American Journal of Physiology. Heart and Circulatory Physiology·S HitchinsG P Dobson
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Jul 3, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Yi JiangEdward W Hsu
May 9, 2002·Biochimica Et Biophysica Acta·Geoffrey P Dobson, Uwe Himmelreich

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