Intracellular ion concentrations and cation-dependent remodelling of bacterial MreB assemblies.

Scientific Reports
Dávid SzatmáriMiklós Nyitrai

Abstract

Here, we measured the concentrations of several ions in cultivated Gram-negative and Gram-positive bacteria, and analyzed their effects on polymer formation by the actin homologue MreB. We measured potassium, sodium, chloride, calcium and magnesium ion concentrations in Leptospira interrogans, Bacillus subtilis and Escherichia coli. Intracellular ionic strength contributed from these ions varied within the 130-273 mM range. The intracellular sodium ion concentration range was between 122 and 296 mM and the potassium ion concentration range was 5 and 38 mM. However, the levels were significantly influenced by extracellular ion levels. L. interrogans, Rickettsia rickettsii and E. coli MreBs were heterologously expressed and purified from E. coli using a novel filtration method to prepare MreB polymers. The structures and stability of Alexa-488 labeled MreB polymers, under varying ionic strength conditions, were investigated by confocal microscopy and MreB polymerization rates were assessed by measuring light scattering. MreB polymerization was fastest in the presence of monovalent cations in the 200-300 mM range. MreB filaments showed high stability in this concentration range and formed large assemblies of tape-like bundles that...Continue Reading

References

Dec 1, 1990·Journal of General Microbiology·A M WhatmoreR H Reed
Sep 24, 1982·Science·P H YanceyG N Somero
May 19, 1998·Trends in Biochemical Sciences·M T RecordH J Guttman
Apr 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·T VoetsB Nilius
May 15, 2002·FEMS Microbiology Reviews·Roy D Sleator, Colin Hill
May 1, 1961·Journal of General Microbiology·J H CHRISTIAN, J A WALTHO
Oct 8, 2004·Molecular Microbiology·Delfina C Dominguez
Nov 19, 2004·The Journal of Biological Chemistry·Osigwe EsueYiider Tseng
Jul 4, 2006·Proceedings of the National Academy of Sciences of the United States of America·Esther Biemans-OldehinkelBert Poolman
Nov 6, 2007·Proceedings of the National Academy of Sciences of the United States of America·Purva Vats, Lawrence Rothfield
Sep 17, 2008·Environmental Microbiology·Lana ShabalaSergey Shabala
Jan 12, 2010·Trends in Microbiology·Waldemar Vollmer, Stephen J Seligman
Mar 18, 2010·Molecular Microbiology·Courtney L WhiteJames W Gober
Jan 29, 2011·International Journal of Pediatric Endocrinology·Richard J AuchusAlan E Zuckerman
Apr 20, 2011·Molecular Microbiology·Leon FurchtgottKerwyn Casey Huang
Jun 4, 2011·Science·Julia Domínguez-EscobarRut Carballido-López
Sep 10, 2011·Proceedings of the National Academy of Sciences of the United States of America·Sven van TeeffelenZemer Gitai
Sep 20, 2011·Journal of Bacteriology·Leyla SlamtiMathieu Picardeau
Oct 4, 2011·The EMBO Journal·Yoshikazu KawaiJeff Errington
Dec 15, 2011·The EMBO Journal·Sven van Teeffelen, Zemer Gitai
Apr 20, 2012·PloS One·Teuta Pilizota, Joshua W Shaevitz
Dec 14, 2012·The Journal of Biological Chemistry·Pearl Nurse, Kenneth J Marians
Feb 20, 2014·Proceedings of the National Academy of Sciences of the United States of America·Alexandre ColavinKerwyn Casey Huang
Mar 8, 2014·Nature Communications·Henrik StrahlLeendert W Hamoen
May 14, 2014·Proceedings of the National Academy of Sciences of the United States of America·Enrique RojasKerwyn Casey Huang
May 21, 2014·ELife·Fusinita van den EntJan Löwe
Aug 6, 2014·Source Code for Biology and Medicine·Guillaume LamourJörg Gsponer
Nov 25, 2014·Biophysical Journal·Teuta Pilizota, Joshua W Shaevitz
Sep 24, 2015·Proceedings of the National Academy of Sciences of the United States of America·Randy M MorgensteinZemer Gitai
Jun 15, 2016·Biochimica Et Biophysica Acta·Szilvia BarkóMiklós Nyitrai
Mar 18, 2020·Nature Communications·Handuo ShiKerwyn Casey Huang

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Datasets Mentioned

BETA
AAS69864.1
ABY73136.1
and
AJF45056.1

Methods Mentioned

BETA
super-resolution microscopy
co-immunoprecipitation
light scattering
Relative light scattering
fluorescence microscopy
Analysis
of
Assay
confocal microscopy

Software Mentioned

Easyworm
mathop

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