Intracellular kinetics of ATX-S10.Na(II) and its correlation with photochemical reaction dynamics during a pulsed photosensitization process: effect of pulse repetition rate

Journal of Biomedical Optics
Satoko KawauchiMakoto Kikuchi

Abstract

Although photodynamic therapy with pulsed light excitation has interesting characteristics, its photosensitization mechanism has not been fully elucidated. In this study, we showed that the intracellular kinetics of ATX-S10.Na(II), a lysosomal sensitizer, was closely related to photochemical reaction dynamics during photodynamic treatment of A549 cells with nanosecond pulsed light. Fluorescence microscopy revealed that at high frequencies of 10 and 30 Hz the sensitizer initially localized mainly in lysosomes but that it started to be redistributed to the cytosol in certain ranges of radiant exposures. These ranges were found to coincide with a regime of fluorescence degradation with limited oxygen consumption. On the other hand, at 5 Hz, there was no such a discontinuous behavior in the sensitizer redistribution characteristics throughout the period of irradiation; this was consistent with the fact that no reaction switching was observed. Two possible reasons for the appearance of the regime with limited oxygen consumption are discussed: participation of an oxygen-independent reaction and change in the microenvironment for the sensitizer caused by lysosomal photodamage. The pulse frequency-dependent intracellular kinetics of th...Continue Reading

References

Feb 1, 1992·Japanese Journal of Cancer Research : Gann·T OkunakaK Aizawa
Dec 1, 1987·Journal of Photochemistry and Photobiology. B, Biology·A Andreoni
Dec 1, 1988·Journal of Photochemistry and Photobiology. B, Biology·F RicchelliL Lindqvist
Apr 1, 1995·Journal of Photochemistry and Photobiology. B, Biology·D KesselK M Smith
Jul 1, 1993·Journal of the American College of Cardiology·N A PragerM E Cain
Feb 1, 1996·Photochemistry and Photobiology·C R LambertI E Kochevar
Mar 1, 1997·Photochemistry and Photobiology·K Berg, J Moan
May 1, 1997·Journal of Photochemistry and Photobiology. B, Biology·M Ochsner
Jun 24, 1998·Journal of the National Cancer Institute·T J DoughertyQ Peng
Jul 29, 1998·Photochemistry and Photobiology·I Georgakoudi, T H Foster
May 30, 2001·Advanced Drug Delivery Reviews·J Morgan, A R Oseroff
Jan 7, 2004·Advanced Drug Delivery Reviews·Anders HøgsetKristian Berg
Dec 1, 2007·Photodiagnosis and Photodynamic Therapy·Patrycja MikolajewskaJohan Moan

❮ Previous
Next ❯

Citations

Sep 1, 2006·Photomedicine and Laser Surgery

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Imaging in CNS

Here is the latest research on cell imaging and imaging modalities, including light-sheet microscopy, in the central nervous system.