PMID: 6468727Jan 1, 1984

Intracellular localization and characterization of 3-hydroxykynureninase in human liver

The International Journal of Biochemistry
J InadaR Kido

Abstract

3-hydroxykynureninase in human liver was present in cytosol and mitochondria. The cytosolic enzyme and mitochondrial enzyme had the same physiological and enzymic properties. The enzyme had a mol. wt of 130,000 by gel filtration and isoelectric point of pH 5.9. The enzyme was active for 3-hydroxykynurenine and kynurenine, and its activity ratio was 15:1. The apparent Km values of the enzyme were 7.7 X 10(-5) M for 3-hydroxykynurenine, 1.0 X 10(-3) M for kynurenine and 2.5 X 10(-6) M for pyridoxal 5'-phosphate with 3-hydroxykynurenine. Some other properties of purified enzymes are described.

References

Jan 1, 1989·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·T IshikawaR Kido
May 5, 2017·International Journal of Tryptophan Research : IJTR·Abdulla A-B Badawy
May 27, 2016·International Journal of Tryptophan Research : IJTR·H BaranB Kepplinger
May 20, 2015·International Journal of Tryptophan Research : IJTR·Katsumi ShibataTsutomu Fukuwatari
Apr 26, 2013·The Journal of Nutrition·Chiaki HiratsukaKatsumi Shibata

Citations

Jul 31, 1973·Biochemistry·M MoriguchiK Soda
Aug 6, 1971·Biochemical and Biophysical Research Communications·M MoriguchiK Soda

Related Concepts

3-hydroxykynureninase
Enzymes, antithrombotic
Kynurenine
Pyridoxal Phosphate
Mitochondria, Liver
Gel Chromatography
Mitochondria
Enzymes for Treatment of Wounds and Ulcers
Hydrolase
Enzymes, hematological

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