PMID: 7019918May 1, 1981Paper

Intracellular localization of DNA polymerase alpha

Proceedings of the National Academy of Sciences of the United States of America
M BrownL M Chang

Abstract

Immunoglobulin (IgG) and the F(ab')2 fragment of IgG were prepared from serum of a rabbit immunized with purified calf thymus DNA polymerase alpha (deoxynucleosidetriphosphate:DNA deoxynucleotidyltransferase, EC 2.7.7.7). An indirect immunofluorescent method based on these reagents was used to detect the intracellular localization of DNA polymerase alpha in primary fetal bovine fibroblasts. The results show that the bulk of DNA polymerase alpha is located in the perinuclear region of the cytoplasm. Immunofluorescent staining of cytoplast and Ficoll-Paque gradient-purified karyoplast fragments resulting from cytochalasin enucleation show the presence of DNA polymerase alpha in cytoplasts and the virtual absence of the enzyme in the nucleus of the karyoplast itself. The implication of this unusual intracellular location for DNA polymerase alpha is discussed.

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Citations

Jan 1, 1991·Experimental Gerontology·V K SrivastavaD Busbee
Aug 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·T J SchmidtG Litwack
Feb 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·L M ChangF J Bollum
Dec 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·J C Cook, P B Chock
Jul 1, 1983·Experimental Cell Research·M Shioda, H Nagano
Jul 29, 1983·Biochemical and Biophysical Research Communications·C S ShashikantM R Das
Apr 1, 1986·Archives of Biochemistry and Biophysics·S N WaltersE T Littledike
Nov 1, 1983·Journal of Cellular Physiology·A MatsukageT Takahashi
Feb 1, 1994·Japanese Journal of Cancer Research : Gann·A WakitaT Kaneda
Jan 1, 1985·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·H H KampingaA W Konings
May 11, 1991·Journal of Endocrinological Investigation·P E BibbinsZ M Lei

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