Intracellular localization of endothelial cell annexins is differentially regulated by oxidative stress

Experimental Cell Research
Sandra M Sacre, Stephen E Moss

Abstract

Annexins are calcium-dependent phospholipid binding proteins that are implicated in the regulation of both intracellular and extracellular thrombostatic mechanisms in the vascular endothelium. Tight control of annexin gene expression and targeting of annexin proteins is therefore of importance in maintaining the health of the endothelium. Because annexins are abundant in vascular endothelial cells and could be either dysregulated by or contribute to anomalies in Ca2+ signaling, we investigated annexin gene expression and subcellular localization in human umbilical vein endothelial cells (HUVEC) in a model of chronic oxidative stress. HUVEC were cultured under mild hyperoxic conditions in a custom-built chamber to induce oxidative stress over a period of 12 days. Although annexin expression levels did not change significantly in response to hyperoxic stress, immunofluorescence analysis revealed striking effects on the subcellular localization of certain annexins, including the redistribution of annexins 5 and 6 from the cytosol to the nucleus. In addition, oxidative stress modulated the responses of certain annexins to stimulation with a range of pharmacological and physiological Ca2+-mobilizing agonists, in a manner that sugges...Continue Reading

Citations

Jun 2, 2005·Nature Reviews. Molecular Cell Biology·Volker GerkeStephen E Moss
Jun 6, 2003·European Journal of Biochemistry·Andreas HofmannAlexander Wlodawer
Oct 14, 2009·Toxicological Sciences : an Official Journal of the Society of Toxicology·Minghua WangDazhi Wang
Feb 28, 2013·International Journal of Molecular Sciences·Lara BoattiFrancesco Marsano
Feb 26, 2013·International Journal of Molecular Sciences·Patrícia A Madureira, David M Waisman
Aug 30, 2002·European Journal of Biochemistry·Lin LiuDavid M Terrian
Apr 22, 2009·Cellular and Molecular Life Sciences : CMLS·Katia MonastyrskayaAnnette Draeger
May 30, 2013·Cellular and Molecular Life Sciences : CMLS·Nina QuiskampUrsula Rescher
Nov 13, 2020·Acta Histochemica Et Cytochemica·Akira IshikawaWataru Yasui
Sep 10, 2014·International Journal for Parasitology·Hayley ToetTerry W Spithill
Nov 26, 2002·Biochimica Et Biophysica Acta·E B BabiychukA Draeger
Oct 7, 2003·International Journal for Parasitology·Malin E-L WeilandStaffan G Svärd

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