PMID: 12786802Jun 6, 2003Paper

Intracellular localization of glycoprotein VI in human platelets and its surface expression upon activation

British Journal of Haematology
Hidenori SuzukiHiroshi Takayama

Abstract

Glycoprotein (GP) VI is a major receptor for collagen and belongs to the immunoglobulin super family. Here, we examined the localization of GPVI in resting and activated human platelets by immunogold scanning and transmission electron microscopy and flow cytometry. Ultrastructural observation detected immunolabelling for GPVI that was distributed uniformly over the entire surface of resting platelets, and revealed that GPVI was also localized on both the membranes of the surface-connected open canalicular system (OCS) and alpha-granules. The OCS- and alpha-granule-associated GPVI pools were an estimated 35.4 +/- 3.2% (mean +/- standard deviation) of the total. Little GPVI labelling was observed in any part of GPVI-deficient platelets. A remarkable time-dependent increase in GPVI surface expression was found by flow cytometry when platelets were activated by collagen-related peptide (CRP) and convulxin. The GPVI-mediated activation of platelets by CRP or convulxin resulted in similar ultrastructural changes and an increased GPVI labelling density on the activated platelet surface, which was accompanied by a decreased interior expression. GPVI was also expressed on microparticles generated from activated platelets. Thus, our stud...Continue Reading

References

Mar 1, 1989·The Histochemical Journal·K T Tokuyasu
Jul 1, 1994·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·A D MichelsonM R Barnard
Jan 1, 1996·Progress in Histochemistry and Cytochemistry·H SuzukiK Tanoue
Oct 25, 2000·Circulation·M MertenP Thiagarajan
Jan 17, 2002·International Journal of Hematology·S Nomura
Aug 22, 2002·Platelets·Maurizio De CaterinaFrancesco Scopacasa

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Citations

Jul 21, 2011·Analytical Biochemistry·Muhammad ZahidPhilippe Billiald
Jun 5, 2008·Molecular Therapy : the Journal of the American Society of Gene Therapy·Tsukasa OhmoriYoichi Sakata
Jul 17, 2004·Current Opinion in Hematology·Olivier MorelJean-Marie Freyssinet
Apr 3, 2008·The Journal of Clinical Investigation·Hiroshi TakayamaShoji Furusako
Dec 24, 2011·Thrombosis·Enma V Paez EspinosaFadi T Khasawneh
Apr 19, 2011·Blood Reviews·Katleen BroosHans Deckmyn
Dec 17, 2014·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Zufar GabbasovPeter Kruzliak
May 20, 2009·Blood Reviews·Price Blair, Robert Flaumenhaft
Dec 9, 2016·Platelets·Oliver PagelRené P Zahedi
Feb 12, 2019·Hypertension·Elena Y SenchenkovaFelicity N E Gavins
Apr 25, 2019·Arteriosclerosis, Thrombosis, and Vascular Biology·Christine Voors-PetteMartine Jandrot-Perrus
Dec 3, 2014·Analytical Chemistry·Secil KoseogluChristy L Haynes
Apr 9, 2018·ACS Biomaterials Science & Engineering·Ujjal Didar Singh Sekhon, Anirban Sen Gupta

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