Intracellular localization of ornithine decarboxylase and its regulatory protein, antizyme-1

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
Raymond G SchipperAlbert A J Verhofstad

Abstract

The enzyme ornithine decarboxylase (ODC) and its regulatory protein antizyme-1 (AZ1) are key regulators in the homeostasis of polyamines. To gain more insight into the exact intracellular distribution of ODC and AZ1, we performed immunocytochemical and Green Fluorescent Protein-fluorocytochemical studies in cultured human cervix carcinoma and human prostatic carcinoma (PC-346C) cells. ODC localization patterns varied from predominantly cytoplasmic to both cytoplasmic and nuclear staining, whereas AZ1 was mostly found in the nucleus. In cells that were synchronized in the mitotic phase, localization of both ODC and AZ1 changed from perinuclear at the beginning of mitosis into nucleoplasmic at close proximity to the chromosomes during meta-, ana- and telophase. Upon completion of mitosis, localization of ODC and AZ1 was reverted back to the cytoplasm, i.e., predominantly perinuclear immediately after cytokinesis. When PC-346C cells were treated with polyamines to induce AZ1-regulated ODC degradation, ODC was predominantly found in the nucleus and colocalized with immunoreactive AZ1. A comparable accumulation of ODC and AZ1 in the nucleus was found in PC-346C cells treated with the polyamine analog SL-11093. The present study sugg...Continue Reading

References

Mar 1, 1986·The Biochemical Journal·A E Pegg
May 26, 1993·Journal of Immunological Methods·R G SchipperA A Verhofstad
Oct 17, 1998·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·K FujiwaraD Tsuru
Nov 2, 1999·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R G SchipperA A Verhofstad
Jul 6, 2000·Molecular Cell·S PyronnetN Sonenberg
Jul 11, 2000·Seminars in Cancer Biology·R G SchipperA A Verhofstad
Mar 10, 2001·Developmental Dynamics : an Official Publication of the American Association of Anatomists·A Gritli-LindeA Linde
Aug 20, 2002·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Raymond G Schipper, Albert A J Verhofstad
Aug 28, 2003·The Journal of Biological Chemistry·Noriyuki MuraiSenya Matsufuji

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Citations

May 13, 2005·Toxicological Sciences : an Official Journal of the Society of Toxicology·C J FongT R Zacharewski
Feb 7, 2006·The Journal of Biological Chemistry·Anthony E Pegg
May 19, 2009·Journal of Cellular Biochemistry·Andrés J López-ContrerasRafael Peñafiel
Feb 10, 2011·Endocrinology·Pavine L C LefèvreBruce D Murphy
Mar 25, 2006·Journal of Experimental Botany·Lenka GemperlováMilena Cvikrová
Apr 29, 2009·Cellular and Molecular Life Sciences : CMLS·Chaim Kahana
Sep 3, 2009·Journal of Cellular Biochemistry·Noriyuki MuraiSenya Matsufuji
Jan 13, 2021·Journal of Cellular Physiology·Yi-Fang YangHui-Chih Hung
Nov 5, 2005·Biochemical and Biophysical Research Communications·Lorin M PetrosJohn F Atkins
May 12, 2009·Experimental Cell Research·Yasuko MurakamiTakami Oka
Mar 2, 2010·Experimental Cell Research·Kristiina KanervaLeif C Andersson

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