Intracellular Metabolic Changes of Rhodococcus sp. LH During the Biodegradation of Diesel Oil

Marine Biotechnology
Ze ChenHao Li

Abstract

In recent years, some marine microbes have been used to degrade diesel oil. However, the exact mechanisms underlying the biodegradation are still poorly understood. In this study, a hypothermophilous marine strain, which can degrade diesel oil in cold seawater was isolated from Antarctic floe-ice and identified and named as Rhodococcus sp. LH. To clarify the biodegradation mechanisms, a gas chromatography-mass spectrometry (GC-MS)-based metabolomics strategy was performed to determine the diesel biodegradation process-associated intracellular biochemical changes in Rhodococcus sp. LH cells. With the aid of partial least squares-discriminant analysis (PLS-DA), 17 differential metabolites with variable importance in the projection (VIP) value greater than 1 were identified. Results indicated that the biodegradation of diesel oil by Rhodococcus sp. LH was affected by many different factors. Rhodococcus sp. LH could degrade diesel oil through terminal or sub-terminal oxidation reactions, and might also possess the ability to degrade aromatic hydrocarbons. In addition, some surfactants, especially fatty acids, which were secreted by Rhodococcus into medium could also assist the strain in dispersing and absorbing diesel oil. Lack of ...Continue Reading

References

Sep 15, 1994·FEMS Microbiology Letters·W AshrafJ C Murrell
Oct 1, 1996·The Analyst·E SepicH Leskovsek
Nov 26, 1998·The Journal of Biological Chemistry·U SchellerW H Schunck
Jan 15, 1999·Critical Reviews in Microbiology·J P SullivanH A Chase
Mar 31, 2004·Molecular Genetics and Metabolism·Fernando ScagliaBrendan Lee
Oct 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Michael P McLeodLindsay D Eltis
Jan 12, 2007·Applied Microbiology and Biotechnology·Jan B van Beilen, Enrico G Funhoff
Mar 10, 2007·Applied Microbiology and Biotechnology·Anett KirschnerUwe T Bornscheuer
Dec 1, 2007·International Journal of Systematic and Evolutionary Microbiology·Jing-Liang XuShun-Peng Li
Sep 17, 2009·Environmental Science and Pollution Research International·Eun-Hee LeeGeum-Sook Hwang
Oct 8, 2009·Environmental Microbiology·Fernando Rojo
Jun 15, 2011·Trends in Microbiology·Catherine L WinderRoyston Goodacre
Jul 17, 2012·Applied and Environmental Microbiology·Marianna A PatrauchanWilliam W Mohn
May 9, 2013·Journal of Bioscience and Bioengineering·Eiji SakuradaniSakayu Shimizu
May 18, 2013·Biotechnology Advances·Yoshihiro Toya, Hiroshi Shimizu
May 27, 2014·Environmental Science and Pollution Research International·Hai-Yan YangLi Li
Sep 9, 2015·Applied Microbiology and Biotechnology·Krisztián LacziGábor Rákhely
Dec 25, 2015·Environmental Science & Technology·Terry C HazenNagissa Mahmoudi

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Aug 19, 2020·AMB Express·Yuki TatemichiMitsuyoshi Ueda

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