PMID: 6535447Jan 1, 1984Paper

Intracellular metabolism of hCG

Annales d'endocrinologie
J L VaitukaitisJ Pehrson

Abstract

After administration of hCG, hormone enters pseudopregnant rat ovarian cells rapidly. Although evidence for small amounts of hCG bound to receptor exists within cells, the major fraction of internalized hCG is free and not receptor bound. The intracellular site of dissociation of hCG from its receptor is unknown. With internalization the hCG molecule is altered so that most of the hormone will no longer bind to concanavalin A. No free hCG alpha or hCG beta was observed for up to 24 hours after internalization. Immunoreactive hCG in cytosol eluted before native highly purified hCG and that altered hCG form may reflect changes in the carbohydrate component of hCG, metabolism of the protein core, or both. Most of the immunoreactive hCG within cytosol remains biologically active for several hours after internalization. The biologic to immunologic (B/I) ratio remained greater than 1.0 for the first six hours after a single injection of hCG. To ascertain whether internalized hCG was predominantly metabolized by lysosomal enzymes in vivo, pseudopregnant rats were pretreated with chloroquine. For the first few hours after hCG stimulation, those animals pretreated with chloroquine attained greater hCG concentrations within the cytosol b...Continue Reading

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