Intracellular monitoring of NADH release from mitochondria using a single functionalized nanowire electrode.

Chemical Science
Hong JiangWei-Hua Huang

Abstract

Mitochondria are the powerhouse of cells, and also their suicidal weapon store. Mitochondrial dysfunction can cause the opening of the mitochondrial permeability transition pore (mPTP) and nicotinamide adenine dinucleotide (NADH) release from mitochondria, eventually leading to the disruption of energy metabolism and even cell death. Hence, NADH is often considered a marker of mitochondrial function, but in situ monitoring of NADH release from mitochondria in single living cells remains a great challenge. Herein, we develop a functionalized single nanowire electrode (NWE) for electrochemical detection of NADH release from intracellular mitochondria by modifying conductive polymer (poly(3,4-ethylendioxythiophene), PEDOT)-coated carbon nanotubes (CNTs) on the surface of a SiC@C nanowire. The positively charged PEDOT facilitates the accumulation of negatively charged NADH at the electrode surface and CNTs promote electron transfer, thus endowing the NWE with high sensitivity and selectivity. Further studies show that resveratrol, a natural product, specifically induced NADH release from mitochondria of MCF-7 cancer cells rather than non-cancerous MCF-10 A cells, indicating the potential therapeutic effects of resveratrol in cancer...Continue Reading

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Citations

Apr 17, 2021·Analytical Methods : Advancing Methods and Applications·Jie ZhangYahong Chen
Jul 24, 2021·Dalton Transactions : an International Journal of Inorganic Chemistry·Chao ZhangQianxiong Zhou
Dec 8, 2020·Analytical Chemistry·Keke HuAndrew G Ewing
Nov 12, 2020·Analytical Chemistry·Juan F Hernández-RodríguezAlberto Escarpa
Nov 19, 2021·Chemical Society Reviews·Wenhui JiWei Huang

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Methods Mentioned

BETA
electrophoresis
Fluorescence imaging
Assay

Software Mentioned

Pulse
Origin Pro

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