Intracellular Osteopontin Induced by CagA-positive Helicobacter pylori Promotes Beta-catenin Accumulation and Interleukin-8 Secretion in Gastric Epithelial cells

Helicobacter
Wei-Lun ChangBor-Shyang Sheu

Abstract

Osteopontin, an important immune modulator and oncogenic promoter, is upregulated in H. pylori-infected gastric mucosa. However, the underlying mechanisms and biological significance are poorly understood. We investigated whether osteopontin was upregulated in gastric epithelial cells by H. pylori and the virulence factors involved. Moreover, cellular component changes caused by osteopontin were also investigated. The gastric epithelial cell line MKN45 was cocultured with wild-type and mutant H. pylori to analyze osteopontin expression. Beta-catenin levels in cell lysate and interleukin-8 levels in supernatant were analyzed. The difference in osteopontin expression levels in both gastric epithelium and plasma was compared between H. pylori-infected patients and uninfected controls. H. pylori induced intracellular, but not secretory, osteopontin expression in MKN45 cells. Accordingly, osteopontin expression intensity in gastric epithelium was higher in H. pylori-infected patients than in controls, but osteopontin levels in plasma were similar between both patient groups. H. pylori virulence factor CagA delivered via the type IV secretion system was essential for intracellular osteopontin upregulation. H. pylori induced β-catenin...Continue Reading

References

Jan 1, 1997·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society· Qu-HongA M Dvorak
Jun 24, 2005·Proceedings of the National Academy of Sciences of the United States of America·Sabine BrandtSteffen Backert
Jul 20, 2005·Proceedings of the National Academy of Sciences of the United States of America·Aime T FrancoRichard M Peek
Dec 22, 2005·Cellular Microbiology·Seok-Yong KimMartin J Blaser
Oct 26, 2007·International Journal of Cancer. Journal International Du Cancer·Yo KurashimaMasanori Hatakeyama
May 16, 2008·Proceedings of the National Academy of Sciences of the United States of America·Mari L ShinoharaHarvey Cantor
Dec 20, 2008·Nature Reviews. Immunology·Harvey Cantor, Mari L Shinohara
Oct 3, 2009·Journal of Cell Communication and Signaling·Susan Amanda LundMarta Scatena
Dec 8, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Makoto InoueMari L Shinohara
Sep 11, 2012·Journal of Cellular Biochemistry·Acacia Lamb, Lin-Feng Chen
Jan 5, 2013·International Journal of Biological Sciences·Xiaojian TangZhenggang Zhu
Feb 22, 2013·Journal of Clinical Gastroenterology·Teng-Yu LeeChun-Ying Wu
Mar 16, 2013·Journal of Cellular and Molecular Medicine·Ravi Thakur, Durga Prasad Mishra
Aug 29, 2013·Cancer Letters·Fei WangLiang Qiao
Dec 24, 2013·World Journal of Gastroenterology : WJG·Ko Eun LeeYoung Do Jung
May 17, 2014·World Journal of Gastroenterology : WJG·Kaname UnoTooru Shimosegawa

❮ Previous
Next ❯

Citations

Jan 19, 2016·Current Opinion in Infectious Diseases·Stephanie L ServetasD Scott Merrell
Mar 30, 2017·Toxins·Olga Sokolova, Michael Naumann

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.

Atrophic Gastritis

Atrophic Gastritis is a process where gastric glandular cells are lost and replaced with firbous tissues, as a result of chronic inflammation. Learn more about Atrophic Gastritis here.