Intracellular Pathways Involved in Bone Regeneration Triggered by Recombinant Silk-silica Chimeras

Advanced Functional Materials
Zaira Martín-MoldesDavid L Kaplan

Abstract

Biomineralization at the organic-inorganic interface is critical to many biology material functions in vitro and in vivo. Recombinant silk-silica fusion peptides are organic-inorganic hybrid material systems that can be effectively used to study and control biologically-mediated mineralization due to the genetic basis of sequence control. However, to date, the mechanisms by which these functionalized silk-silica proteins trigger the differentiation of human mesenchymal stem cells (hMSCs) to osteoblasts remain unknown. To address this challenge, we analyzed silk-silica surfaces for silica-hMSC receptor binding and activation, and the intracellular pathways involved in the induction of osteogenesis on these bioengineered biomaterials. The induction of gene expression of αVβ3 integrin, all three Mitogen-activated Protein Kinsases (MAPKs) as well as c-Jun, Runt-related Transcription Factor 2 (Runx2) and osteoblast marker genes was demonstrated upon growth of the hMSCs on the silk-silica materials. This induction of key markers of osteogenesis correlated with the content of silica on the materials. Moreover, computational simulations were performed for silk/silica-integrin binding which showed activation of αVβ3 integrin in contact ...Continue Reading

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Citations

Oct 31, 2018·Macromolecular Bioscience·Diego López BarreiroMarkus J Buehler
Nov 16, 2019·Journal of Functional Biomaterials·Rachael N ParkerDavid L Kaplan
Nov 20, 2018·Scientific Reports·D V ScholeyC C Perry
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Jun 11, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Chenxi ZhaiJingjie Yeo
Jul 2, 2021·Biophysical Journal·Jazmin OzsvarAnthony S Weiss
Dec 24, 2020·ACS Applied Materials & Interfaces·Xiaomeng GaoQianbing Wan
Jan 20, 2021·ACS Biomaterials Science & Engineering·Vladimir G Debabov, Vladimir G Bogush

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