PMID: 8583280Dec 1, 1995Paper

Intracellular pH changes induced in Propionibacterium acnes by UVA radiation and blue light

Journal of Photochemistry and Photobiology. B, Biology
C M FutsaetherA Johnsson

Abstract

The intracellular pH changes induced in the Gram-positive skin bacterium Propionibacterium acnes by blue light and UVA radiation were studied. Two methods (31P nuclear magnetic resonance (NRM) spectroscopy and fluorescence spectroscopy using a pH-sensitive fluoroprobe (2',7'-bis-(2-carboxyethyl)-5-(and -6-)-carboxyfluorescein)) were used to determine the intracellular pH. The pH changes induced by irradiation were found to be a function of cell survival. These changes as a function of cell survival followed the same pattern for blue light and UVA radiation. A reduction of the pH gradient across the cell membrane (inside alkaline) was found for lethal doses (less than 15% survival). This reduction corresponded to a decrease in intracellular pH and may indicate a proton influx. An increase in the pH gradient, which corresponded to an increase in the intracellular pH, was observed for sublethal doses. This increase appears to be reversible. Thus two separate mechanisms, which appear to be the same in UVA and blue light regions, may be responsible for the irradiation-induced pH alterations.

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Citations

May 16, 2000·The British Journal of Dermatology·P PapageorgiouA Chu
May 1, 2001·International Journal of Dermatology·C N Burkhart
Aug 11, 2004·American Journal of Clinical Dermatology·Aikaterini CharakidaAntony C Chu
Sep 16, 2008·Journal of Cosmetic Dermatology·Sadia AmmadCaroline Mills
Nov 18, 2006·Lasers in Surgery and Medicine·Seung Yoon LeeMi Youn Park
Apr 25, 2009·Journal of Cosmetic and Laser Therapy : Official Publication of the European Society for Laser Dermatology·Neil Sadick
Jan 6, 2006·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Ståle RamstadAnders Johnsson
May 23, 2013·Environmental Science & Technology·Ana L SantosAngela Cunha
Jul 15, 2004·Advances in Applied Microbiology·Robert H Reed
Oct 27, 2005·The Journal of Dermatological Treatment·C A MortonJ Birch
Feb 23, 2019·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Hyuck Hoon KwonGyeong-Hun Park
Oct 15, 2010·Anais Brasileiros De Dermatologia·Maria Cláudia Almeida Issa, Mônica Manela-Azulay

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