PMID: 8611181Feb 1, 1996Paper

Intracellular pH governs the subcellular distribution of hexokinase in a glioma cell line

The Biochemical Journal
L MiccoliM F Poupon

Abstract

Hexokinase plays a key role in regulating cell energy metabolism. Hexokinase is mainly particulate, bound to the mitochondrial outer membrane in brain and tumour cells. We hypothesized that the intracellular pH (pH1) controls the intracellular distribution of hexokinase. Using the SNB-19 glioma cell line, pH1 variations were imposed by incubating cells in a high-K+ medium at different pH values containing specific ionophores (nigericin and valinomycin), without affecting cell viability. Subcellular fractions of cell homogenates were analysed for hexokinase activity. Imposed pH1 changes were verified microspectrofluorimetrically by using the pH1-sensitive probe SNARF-1-AM (seminaphtho-rhodafluor-1-acetoxymethyl ester). Imposition of an acidic pH1 for 30 min strongly decreased the particulate/total hexokinase ratio, from 63% in the control sample to 31%. Conversely, when a basic pH1, was imposed, the particulate/total hexokinase ratio increased to 80%. The glycolytic parameters, namely lactate/pyruvate ratio, glucose 6-phosphate and ATP levels, were measured concomitantly. Lactate/pyruvate ratio and ATP level were both markedly decreased by acidic pH1 and increased by basic pH1. Conversely, the glucose 6-phosphate level was incre...Continue Reading

Citations

Dec 20, 2008·Biochimica Et Biophysica Acta·Varda Shoshan-BarmatzSalah Abu-Hamad
Jan 16, 2017·Seminars in Cancer Biology·Kévin HardonnièreDominique Lagadic-Gossmann
Sep 25, 1998·Journal of the National Cancer Institute·L MiccoliS Oudard
Mar 27, 2018·Acta Physiologica·M FlinckS F Pedersen
Feb 17, 2017·Journal of Cell Science·Katharine A WhiteDiane L Barber
Feb 12, 2008·Journal of Cellular Physiology·Sara Rodríguez-EnríquezRafael Moreno-Sánchez
Nov 2, 2018·International Urology and Nephrology·Jonathan BloomSensuke Konno
Mar 11, 2004·The Journal of Pharmacology and Experimental Therapeutics·Manu HegdeFredric Gorin
Sep 12, 2019·Physiological Reviews·S F Pedersen, L Counillon
Feb 6, 2002·Melanoma Research·P R WachsbergerD B Leeper
Feb 24, 2007·Biotechnology and Bioengineering·Shinya TakumaJames M Piret
Feb 7, 2001·American Journal of Physiology. Cell Physiology·L A McLeanP M Cala
Aug 9, 2020·OncoTargets and Therapy·Wei HanWei Guan
Jan 29, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Guanzhuang GaoQiaoming Zhi
Jul 18, 2008·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Rituka I Sharma, Tim A D Smith

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