Intracellular pH in arbuscular mycorrhizal fungi. A symbiotic physiological marker

Plant Physiology
M JolicoeurGuillaume Bécard

Abstract

A method was developed to perform real-time analysis of cytosolic pH of arbuscular mycorrhizal fungi in culture using dye and ratiometric measurements (490/450 nm excitations). The study was mainly performed using photometric analysis, although some data were confirmed using image analysis. The use of nigericin allowed an in vivo calibration. Experimental parameters such as loading time and concentration of the dye were determined so that pH measurements could be made for a steady-state period on viable cells. A characteristic pH profile was observed along hyphae. For Gigaspora margarita, the pH of the tip (0-2 &mgr;m) was typically 6.7, increased sharply to 7.0 behind this region (9.5 &mgr;m), and decreased over the next 250 &mgr;m to a constant value of 6.6. A similar pattern was obtained for Glomus intraradices. The pH profile of G. margarita germ tubes was higher when cultured in the presence of carrot (Daucus carota) hairy roots (nonmycorrhizal). Similarly, extraradical hyphae of G. intraradices had a higher apical pH than the germ tubes. The use of a paper layer to prevent the mycorrhizal roots from being in direct contact with the medium selected hyphae with an even higher cytosolic pH. Results suggest that this method c...Continue Reading

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Citations

Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Maria J. Harrison
Aug 7, 2010·Annual Review of Phytopathology·Xiaonan XieKoichi Yoneyama
Apr 2, 2005·Biotechnology Progress·Steve Hisiger, Mario Jolicoeur
Dec 11, 2007·Journal of Environmental Radioactivity·H Dupré de BouloisS Declerck
Jan 23, 2008·The New Phytologist·Alessandro C RamosJosé A Feijó
Nov 1, 2001·Fungal Genetics and Biology : FG & B·J Wendland
Aug 26, 2009·Plant Signaling & Behavior·Alessandro C RamosJosé A Feijó
Mar 27, 2012·FEMS Yeast Research·Citlali Fonseca-GarcíaJosé Ruiz-Herrera
Oct 3, 2007·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Siegrid SteinkellnerHorst Vierheilig
Sep 2, 2017·Journal of Experimental Botany·Octavian O H Stephan
Nov 30, 2020·The Science of the Total Environment·E Gil-PachecoA García-Sánchez
Sep 1, 2004·The New Phytologist·David L JonesYakov Kuzyakov

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