PMID: 10614Oct 1, 1976

Intracellular pH in cold-blooded vertebrates as a function of body temperature

Respiration Physiology
A MalanR B Reeves

Abstract

Intracellular pH (pHi) was measured in vivo in tissue of frogs (Rana catesbeiana) and turtles (Pseudemys scripta) using the DMO technique. Animals were permitted 3-8 days to come to a new steady-state body temperature (Tb) which ranged 5-32 degrees C. Least squares regression equation for pHi data are: frog blood, 8.184-0.0206 Tb; frog striated muscle, 7.275-0.0152 Tb; turtle blood, 8.092-0.0207Tb; turtle muscle, 7.421-0.0186 Tb; turtle heart, 7.452-0.0122 Tb; turtle liver, 7.753-0.0233 Tb; turtle esophageal smooth muscle, 7.513-0.0141 Tb. Only turtle cardiac muscle deltapHi/deltaT was significantly different from deltapH/deltaT of blood. Results have been interpreted in terms of protein charge state alterations; in the physiological pH range, histidine residues of proteins are the principal dissociable groups (HPr+ = H+ + Pr) affected by pHi and Tb changes. Constancy of protein charge state can be assessed by monitoring alpha imidazole, alphaIM = Pr/(HPr+ + Pr). A uniform pKIM of 6.85 (20degreesC) and a deltaHO of 7 kcal/mol are assumed in calculating alphaIM. Intracellular alphaIM is preserved in the tissues studied as body temperature changes. These results indicate that ectotherm acid-base balance, alphastat control, regula...Continue Reading

References

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Citations

Oct 1, 1976·Respiration Physiology·R B Reeves, A Malan
Dec 1, 1980·Respiration Physiology·J L Rodeau, A Malan
Jan 1, 1983·Comparative Biochemistry and Physiology. A, Comparative Physiology·Y S ParkS K Hong
Jan 1, 1986·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·C DoumenR Blust
Jan 1, 1986·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·A IshimatsuS Nilsson
Jan 1, 1989·Annals of Emergency Medicine·K A DelaneyL R Goldfrank
Sep 29, 1999·Progress in Neurobiology·E Nattie
May 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·A R Place, D A Powers
Aug 5, 2014·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Christian Lind MalteTobias Wang
Mar 4, 2014·Integrative and Comparative Biology·A Malan
Mar 1, 1988·General and Comparative Endocrinology·S F PerryT W Moon
May 29, 2003·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Maria ErecinskaIan A Silver
May 1, 1993·The Journal of Experimental Zoology·J S WasserS J Warburton
Jan 9, 1999·The Journal of Biological Chemistry·C F BonafeM S Matsuura
Mar 31, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Daniel E Warren, Donald C Jackson
Jan 1, 1995·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·D C JacksonJ S Wasser
Jan 1, 1985·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·A MalanF Daull
Aug 12, 2019·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Anne B Kim, William K Milsom

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