PMID: 37742Jul 1, 1979

Intracellular pH measurements in gastric mucosa

The American Journal of Physiology
S J Hersey

Abstract

Intracellular pH was measured in bullfrog gastric mucosa using a pH-indicator dye, bromthymol blue (BTB), with a spectrophotometric technique. Studies showed that BTB is taken up by the gastric mucosa and bound to intracellular components. The binding of BTB was shown to cause a shift in the pKa of the dye from the solution value of 6.95 to a value of 8.0. During the nonsecreting state, intracellular pH was estimated to be 7.4 (metiamide inhibition) or 7.1 (SCN inhibition). During active secretion of acid, intracellular pH increased with increasing secretion rates, reaching values in excess of pH 8. Using preparations from which the surface epithelial cells had been removed, it was shown that at least a portion of the alkaline response to stimulation occurs in the oxyntic or tubular cells. The results are interpreted in view of existing models for the chemical reaction involved in gastric acid secretion.

References

Aug 1, 1976·The Journal of General Physiology·V W MacDonald, F F Jöbsis
Oct 1, 1977·Physiological Reviews·J L Walker, H M Brown
Jan 1, 1978·The Journal of Cell Biology·K S CarlisleS J Hersey
Sep 16, 1974·Biochimica Et Biophysica Acta·S J Hersey
Oct 1, 1972·The American Journal of Physiology·S J Hersey, W L High
Aug 20, 1971·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·S J Hersey
Dec 11, 1973·The American Journal of Physiology·S S SandersW S Rehm
Jun 1, 1974·The American Journal of Physiology·W L High, S J Hersey
May 1, 1972·Journal of Applied Physiology·J G ForteJ L Poulter
Jun 1, 1971·Biochimica Et Biophysica Acta·S J Hersey, W L High
May 20, 1958·The Journal of General Physiology·R P DURBIN, E HEINZ
Jul 1, 1951·The Journal of Physiology·T TEORELL
Feb 1, 1951·Biological Reviews of the Cambridge Philosophical Society·R E DAVIES

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